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首页> 外文期刊>Molecular and Cellular Biology >Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p.
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Transcriptional corepression in vitro: a Mot1p-associated form of TATA-binding protein is required for repression by Leu3p.

机译：体外转录共抑制：Leu3p抑制需要与Mot1p相关的TATA结合蛋白形式。

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摘要

Signals from transcriptional activators to the general mRNA transcription apparatus are communicated by factors associated with RNA polymerase II or the TATA-binding protein (TBP). Currently, little is known about how gene-specific transcription repressors communicate with RNA polymerase II. We have analyzed the requirements for repression by the saccharomyces cerevisiae Leu3 protein (Leu3p) in a reconstituted transcription system. We have identified a complex form of TBP which is required for communication of the repressing signal. This TFIID-like complex contains a known TBP-associated protein, Mot1p, which has been implicated in the repression of a subset of yeast genes by genetic analysis. Leu3p-dependent repression can be reconstituted with purified Mot1p and recombinant TBP. In addition, a mutation in the Mot1 gene leads to partial derepression of the Leu3p-dependent LEU2 promoter. These in vivo and in vitro observations define a role for Mot1p as a transcriptional corepressor.
机译：从转录激活因子到一般mRNA转录装置的信号通过与RNA聚合酶II或TATA结合蛋白（TBP）相关的因子传递。目前，关于基因特异性转录阻遏物如何与RNA聚合酶II通讯的了解甚少。我们已经分析了在重组转录系统中酿酒酵母Leu3蛋白（Leu3p）抑制的要求。我们已经确定了抑制信号的传递所需的TBP的复杂形式。这种类似TFIID的复合物包含一种已知的与TBP相关的蛋白Mot1p，该蛋白已通过基因分析与抑制酵母基因的一个子集有关。可以用纯化的Mot1p和重组TBP重建Leu3p依赖性阻抑。此外，Mot1基因中的突变导致依赖Leu3p的LEU2启动子部分抑制。这些体内和体外的观察结果定义了Mot1p作为转录共抑制因子的作用。

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