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首页> 外文期刊>Molecular and Cellular Biology >Functional domains of the transcription factor USF2: atypical nuclear localization signals and context-dependent transcriptional activation domains.
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Functional domains of the transcription factor USF2: atypical nuclear localization signals and context-dependent transcriptional activation domains.

机译：转录因子USF2的功能域：非典型核定位信号和上下文相关的转录激活域。

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摘要

USF is a family of basic helix-loop transcriptional factors that recognizes DNA-binding sites similar to those of the Myc oncoproteins. Here, various functional domains in the mouse USF2 protein were identified and characterized. Indirect immunofluorescence studies with transiently transfected cells revealed that both the basic region and the highly conserved USF-specific region (USR) are involved in the nuclear localization of USF2. Cotransfection assays with deletion mutants containing the DNA-binding domain of either USF2 or GAL4 identified two distinct transcriptional activation domains in USF2, the USR and the exon 5-encoded region. Activity of the exon 5 activation domain was detectable in both assay systems. Within USF2, however, its potency varied with the conformation induced by the surrounding regions, especially that encoded by alternatively spliced exon 4. In contrast, the USR activated transcription only in its natural context upstream of the USF2 basic region and only with reporter constructs containing the adenovirus major late minimal promoter but not the E1b minimal promoter. However, insertion of an initiator element downstream of the TATA box rescued the activity of the USR on the E1b-driven reporters. The USR therefore represents a new type of activation domain whose function depends very strongly on the core promoter context.
机译：USF是一个基本的螺旋环转录因子家族，可识别与Myc癌蛋白相似的DNA结合位点。在这里，小鼠USF2蛋白中的各种功能域已被鉴定和表征。用瞬时转染的细胞进行的间接免疫荧光研究表明，基本区域和高度保守的USF特异性区域（USR）都参与了USF2的核定位。使用含有USF2或GAL4的DNA结合结构域的缺失突变体进行的共转染测定，在USF2，USR和外显子5编码区中发现了两个不同的转录激活结构域。在两个测定系统中均可检测到外显子5激活域的活性。但是，在USF2中，其效力随周围区域诱导的构象而变化，尤其是由交替剪接的外显子4编码的构象。相反，USR仅在其自然背景下在USF2基本区域上游激活转录，并且仅与包含腺病毒主要晚期最小启动子，而不是E1b最小启动子。但是，在TATA盒下游插入引发剂元件可以挽救USR在E1b驱动的报道分子上的活性。因此，USR代表了一种新型的激活域，其功能在很大程度上取决于核心启动子的环境。

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