Id3 prevents differentiation of preadipose cells.

M Moldes; F Lasnier; B Fève; J Pairault; P Djian

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Id3 prevents differentiation of preadipose cells.

机译：Id3防止脂肪细胞分化。

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We have studied the expression of the Id1, Id2, and Id3 genes during adipose differentiation of 3T3-F442A cells. All three Id mRNAs are present in preadipose cells, but the mRNA for Id3 is the most abundant. All three Id mRNAs sharply decline in the course of adipose differentiation, and their virtual disappearance precedes differentiation. The decrease in Id2 and Id3 is associated with adipose differentiation rather than with growth arrest since it is not observed in 3T3-C2 cells, a fibroblast line with a very low susceptibility to adipose conversion. The decline in Id2 and Id3 mRNAs is associated with a reduced transcription rate of the two genes. Id1 mRNA is reduced in amount during adipose conversion of 3T3-F442A cells, but the decrease is also observed in resting 3T3-C2 cells and is associated with very little decrease in transcription of the gene. Addition of fresh serum reactivates Id3 gene expression in quiescent 3T3-C2 cells but not in adipose 3T3-F442A cells. Stably transformed preadipose cells expressing an Id3 cDNA under the control of a viral promoter are virtually unable to differentiate. We postulate that the Id3 protein is a negative regulator of fat cell formation and presumably acts by preventing an as yet unidentified basic helix-loop-helix protein from activating the program of differentiation.

机译：我们已经研究了3T3-F442A细胞脂肪分化过程中Id1，Id2和Id3基因的表达。所有三个Id mRNA均存在于脂肪细胞中，但Id3的mRNA最丰富。在脂肪分化过程中，所有三个Id mRNA均急剧下降，并且它们的虚拟消失先于分化。 Id2和Id3的减少与脂肪分化有关，而不与生长停滞有关，因为在3T3-C2细胞（一种对脂肪转化的敏感性非常低的成纤维细胞系）中没有观察到它。 Id2和Id3 mRNA的下降与两个基因的转录速率降低有关。在3T3-F442A细胞的脂肪转化过程中，Id1 mRNA的量减少了，但是在静止的3T3-C2细胞中也观察到了该量的减少，并且与基因转录的减少几乎没有关系。新鲜血清的添加可在静止的3T3-C2细胞中重新激活Id3基因表达，而在脂肪3T3-F442A细胞中则不会。在病毒启动子的控制下，稳定转化的表达Id3 cDNA的脂肪细胞实际上无法分化。我们假设Id3蛋白是脂肪细胞形成的负调节剂，并可能通过阻止尚未确定的基本螺旋-环-螺旋蛋白激活分化程序而发挥作用。

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《Molecular and Cellular Biology》 |1997年第4期|共9页
作者
M Moldes; F Lasnier; B Fève; J Pairault; P Djian;
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中图分类细胞生物学;
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6. Id3 prevents differentiation of preadipose cells. [O] . M Moldes, F Lasnier, B Fève, 1997

机译：Id3防止脂肪细胞分化。
7. Id3 prevents differentiation of preadipose cells. [O] . Moldes, M, Lasnier, F, Fève, B, 1997

机译：Id3防止脂肪细胞分化。

Id3 prevents differentiation of preadipose cells.

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