• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >RRB1 and RRB2 encode maize retinoblastoma-related proteins that interact with a plant D-type cyclin and geminivirus replication protein.
【24h】

RRB1 and RRB2 encode maize retinoblastoma-related proteins that interact with a plant D-type cyclin and geminivirus replication protein.

机译：RRB1和RRB2编码与植物D型细胞周期蛋白和双生病毒复制蛋白相互作用的玉米成视网膜细胞瘤相关蛋白。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Unlike mammalian and yeast cells, little is known about how plants regulate G1 progression and entry into the S phase of the cell cycle. In mammalian cells, a key regulator of this process is the retinoblastoma tumor suppressor protein (RB). In contrast, G1 control in Saccharomyces cerevisiae does not utilize an RB-like protein. We report here the cloning of cDNAs from two Zea mays genes, RRB1 and RRB2, that encode RB-related proteins. Further, RRB2 transcripts are alternatively spliced to yield two proteins with different C termini. At least one RRB gene is expressed in all the tissues examined, with the highest levels seen in the shoot apex. RRB1 is a 96-kDa nuclear protein that can physically interact with two mammalian DNA tumor virus oncoproteins, simian virus 40 large-T antigen and adenovirus E1A, and with a plant D-type cyclin. These associations are abolished by mutation of a conserved cysteine residue in RRB1 that is also essential for RB function. RRB1 binding potential is also sensitive to deletions in the conserved A and B domains, although differences exist in these effects compared to those of human RB. RRB1 can also bind to the AL1 protein from tomato golden mosaic virus (TGMV), a protein which is essential for TGMV DNA replication. These results suggest that G1 regulation in plant cells is controlled by a mechanism which is much more similar to that found in mammalian cells than that in yeast.
机译：与哺乳动物和酵母细胞不同，关于植物如何调节G1进程以及进入细胞周期S期的知之甚少。在哺乳动物细胞中，该过程的关键调节因子是成视网膜细胞瘤抑癌蛋白（RB）。相反，酿酒酵母中的G1对照不利用RB样蛋白。我们在这里报告了从两个玉米基因RRB1和RRB2的cDNA克隆，该基因编码RB相关蛋白。此外，剪接RRB2转录物以产生具有不同C末端的两种蛋白质。在所检查的所有组织中至少表达一种RRB基因，其中最高的水平出现在茎尖。 RRB1是一种96 kDa的核蛋白，可以与两种哺乳动物DNA肿瘤病毒癌蛋白，猿猴病毒40大T抗原和腺病毒E1A以及植物D型细胞周期蛋白发生物理相互作用。 RRB1中保守的半胱氨酸残基的突变消除了这些关联，这对于RB功能也是必不可少的。 RRB1的结合潜力也对保守的A和B结构域中的删除敏感，尽管与人类RB相比，这些影响存在差异。 RRB1还可以与番茄金黄花叶病毒（TGMV）的AL1蛋白结合，该蛋白是TGMV DNA复制所必需的。这些结果表明，植物细胞中的G1调控受一种机制控制，该机制与哺乳动物细胞中的机制比酵母中的机制更为相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号