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首页> 外文期刊>Molecular and Cellular Biology >Targeted Gene Knockout Reveals a Role in Meiotic Recombination for ZHP-3, a Zip3-Related Protein in Caenorhabditis elegans
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Targeted Gene Knockout Reveals a Role in Meiotic Recombination for ZHP-3, a Zip3-Related Protein in Caenorhabditis elegans

机译：靶向基因敲除揭示了秀丽隐杆线虫ZHP3相关蛋白ZHP-3在减数分裂重组中的作用。

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The meiotically expressed Zip3 protein is found conserved from Saccharomyces cerevisiae to humans. In baker's yeast, Zip3p has been implicated in synaptonemal complex (SC) formation, while little is known about the protein's function in multicellular organisms. We report here the successful targeted gene disruption of zhp-3 (K02B12.8), the ZIP3 homolog in the nematode Caenorhabditis elegans. Homozygous zhp-3 knockout worms show normal homologue pairing and SC formation. Also, the timing of appearance and the nuclear localization of the recombination protein Rad-51 seem normal in these animals, suggesting proper initiation of meiotic recombination by DNA double-strand breaks. However, the occurrence of univalents during diplotene indicates that C. elegans ZHP-3 protein is essential for reciprocal recombination between homologous chromosomes and thus chiasma formation. In the absence of ZHP-3, reciprocal recombination is abolished and double-strand breaks seem to be repaired via alternative pathways, leading to achiasmatic chromosomes and the occurrence of univalents during meiosis I. Green fluorescent protein-tagged C. elegans ZHP-3 forms lines between synapsed chromosomes and requires the SC for its proper localization.
机译：发现减数分裂表达的Zip3蛋白从酿酒酵母（Saccharomyces cerevisiae）对人而言是保守的。在面包酵母中，Zip3p与突触复合物（SC）的形成有关，而对该蛋白在多细胞生物中的功能知之甚少。我们在这里报告线虫秀丽隐杆线虫 ZIP3 同源物 zhp-3 （K02B12.8）的成功靶向基因破坏。纯合子 zhp-3 敲除蠕虫显示出正常的同源物配对和SC形成。同样，重组蛋白Rad-51的出现时间和核定位在这些动物中似乎是正常的，这表明通过DNA双链断裂适当地启动了减数分裂重组。然而，在二戊烯期间单价的出现表明 C。秀丽线虫ZHP-3蛋白对于同源染色体之间的相互重组以及由此形成的裂痕是必不可少的。在没有ZHP-3的情况下，相互的重组被废除了，双链断裂似乎通过替代途径得以修复，从而导致染色体趋于分裂，并在减数分裂I期间出现单价。绿色荧光蛋白标记的 C。秀丽线虫ZHP-3在突触的染色体之间形成线，需要SC才能正确定位。

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