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首页> 外文期刊>Molecular and Cellular Biology >The ctf13-30/CTF13 Genomic Haploinsufficiency Modifier Screen Identifies the Yeast Chromatin Remodeling Complex RSC, Which Is Required for the Establishment of Sister Chromatid Cohesion
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The ctf13-30/CTF13 Genomic Haploinsufficiency Modifier Screen Identifies the Yeast Chromatin Remodeling Complex RSC, Which Is Required for the Establishment of Sister Chromatid Cohesion

机译：ctf13-30 / CTF13基因组单倍型不足修饰符屏幕可确定酵母染色质重塑复合物RSC，这是建立姐妹染色单体凝聚力所必需的

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The budding yeast centromere-kinetochore complex ensures high-fidelity chromosome segregation in mitosis and meiosis by mediating the attachment and movement of chromosomes along spindle microtubules. To identify new genes and pathways whose function impinges on chromosome transmission, we developed a genomic haploinsufficiency modifier screen and used ctf13-30, encoding a mutant core kinetochore protein, as the reference point. We demonstrate through a series of secondary screens that the genomic modifier screen is a successful method for identifying genes that encode nonessential proteins required for the fidelity of chromosome segregation. One gene isolated in our screen was RSC2, a nonessential subunit of the RSC chromatin remodeling complex. rsc2 mutants have defects in both chromosome segregation and cohesion, but the localization of kinetochore proteins to centromeres is not affected. We determined that, in the absence of RSC2, cohesin could still associate with chromosomes but fails to achieve proper cohesion between sister chromatids, indicating that RSC has a role in the establishment of cohesion. In addition, numerous subunits of RSC were affinity purified and a new component of RSC, Rtt102, was identified. Our work indicates that only a subset of the nonessential RSC subunits function in maintaining chromosome transmission fidelity.
机译：出芽的酵母着丝粒-线粒体复合体通过介导染色体沿着纺锤体微管的附着和运动，确保有丝分裂和减数分裂中的高保真染色体分离。为了鉴定功能影响染色体传递的新基因和途径，我们开发了基因组单倍体不足修饰物筛选器，并使用编码突变核心动粒蛋白的 ctf13-30 作为参考点。我们通过一系列的辅助筛选证明，基因组修饰子筛选是一种成功的方法，可用于鉴定编码染色体分离保真度所需的非必需蛋白的基因。在我们的筛选中分离出的一个基因是 RSC2 ，这是RSC染色质重塑复合体的非必需亚基。 rsc2 突变体在染色体分离和内聚性上均存在缺陷，但动粒体蛋白在着丝粒上的定位不受影响。我们确定，在没有 RSC2 的情况下，粘着蛋白仍可与染色体结合，但无法在姐妹染色单体之间实现适当的粘着，这表明RSC在建立粘着力中起作用。此外，亲和纯化了RSC的许多亚基，并鉴定了RSC的新组件Rtt102。我们的工作表明，非必需RSC亚基的一个子集在维持染色体传递保真度中起作用。

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