• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Activation of the G2/M-Specific Gene CLB2 Requires Multiple Cell Cycle Signals
【24h】

Activation of the G2/M-Specific Gene CLB2 Requires Multiple Cell Cycle Signals

机译：G2 / M特异性基因CLB2的激活需要多个细胞周期信号

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In budding yeast (Saccharomyces cerevisiae), the periodic expression of the G2/M-specific gene CLB2 depends on a DNA binding complex that mediates its repression during G1 and activation from the S phase to the exit of mitosis. The switch from low to high expression levels depends on the transcriptional activator Ndd1. We show that the inactivation of the Sin3 histone deacetylase complex bypasses the essential role of Ndd1 in cell cycle progression. Sin3 and its catalytic subunit Rpd3 associate with the CLB2 promoter during the G1 phase of the cell cycle. Both proteins dissociate from the promoter at the onset of the S phase and reassociate during G2 phase. Sin3 removal coincides with a transient increase in histone H4 acetylation followed by the expulsion of at least one nucleosome from the promoter region. Whereas the first step depends on Cdc28/Cln1 activity, Ndd1 function is required for the second step. Since the removal of Sin3 is independent of Ndd1 recruitment and Cdc28/Clb activity it represents a unique regulatory step which is distinct from transcriptional activation.
机译：在发芽酵母中（ Saccharomyces cerevisiae ），G 2 / M特异性基因 CLB2 的周期表达取决于介导的DNA结合复合物。其在G 1 期间的抑制和从S期到有丝分裂退出的激活。从低表达水平到高表达水平的转换取决于转录激活因子Ndd1。我们表明，Sin3组蛋白脱乙酰酶复合物的失活绕过Ndd1在细胞周期进程中的基本作用。 Sin3及其催化亚基Rpd3在细胞周期的G 1 阶段与 CLB2 启动子结合。两种蛋白都在S期开始时与启动子分离，并在G 2 期重新结合。 Sin3的去除与组蛋白H4乙酰化的短暂增加相吻合，随后从启动子区域排出至少一个核小体。第一步取决于Cdc28 / Cln1活性，而第二步则需要Ndd1功能。由于Sin3的去除与Ndd1募集和Cdc28 / Clb活性无关，因此它代表了不同于转录激活的独特调节步骤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号