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首页> 外文期刊>Molecular and Cellular Biology >The Transcriptional Coactivator SAYP Is a Trithorax Group Signature Subunit of the PBAP Chromatin Remodeling Complex
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The Transcriptional Coactivator SAYP Is a Trithorax Group Signature Subunit of the PBAP Chromatin Remodeling Complex

机译：转录共激活因子SAYP是PBAP染色质重塑复合体的Trithorax基团签名亚基。

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SWI/SNF ATP-dependent chromatin remodeling complexes (remodelers) perform critical functions in eukaryotic gene expression control. BAP and PBAP are the fly representatives of the two evolutionarily conserved major subclasses of SWI/SNF remodelers. Both complexes share seven core subunits, including the Brahma ATPase, but differ in a few signature subunits; POLYBROMO and BAP170 specify PBAP, whereas OSA defines BAP. Here, we show that the transcriptional coactivator and PHD finger protein SAYP is a novel PBAP subunit. Biochemical analysis established that SAYP is tightly associated with PBAP but absent from BAP. SAYP, POLYBROMO, and BAP170 display an intimately overlapping distribution on larval salivary gland polytene chromosomes. Genome-wide expression analysis revealed that SAYP is critical for PBAP-dependent transcription. SAYP is required for normal development and interacts genetically with core- and PBAP-selective subunits. Genetic analysis suggested that, like BAP, PBAP also counteracts Polycomb silencing. SAYP appears to be a key architectural component required for the integrity and association of the PBAP-specific module. We conclude that SAYP is a signature subunit that plays a major role in the functional specificity of the PBAP holoenzyme.
机译：SWI / SNF ATP依赖的染色质重塑复合物（重塑剂）在真核基因表达控制中起关键作用。 BAP和PBAP是SWI / SNF重塑器的两个进化上保守的主要亚类的动态代表。两种复合物共有七个核心亚基，包括梵天ATPase，但在几个特征性亚基上有所不同。 POLYBROMO和BAP170指定PBAP，而OSA定义BAP。在这里，我们表明转录共激活因子和PHD手指蛋白SAYP是一种新型的PBAP亚基。生化分析确定SAYP与PBAP紧密相关，但BAP则不存在。 SAYP，POLYBROMO和BAP170在幼虫唾液腺多烯染色体上显示出紧密重叠的分布。全基因组表达分析表明SAYP对于依赖PBAP的转录至关重要。 SAYP是正常发育所必需的，并与核心和PBAP选择性亚基发生遗传相互作用。遗传分析表明，与BAP一样，PBAP也可以抵消Polycomb沉默。 SAYP似乎是PBAP特定模块的完整性和关联所必需的关键体系结构组件。我们得出的结论是SAYP是一个签名亚基，在PBAP全酶的功能特异性中起主要作用。

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