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首页> 外文期刊>Molecular and Cellular Biology >Nap1 Links Transcription Elongation, Chromatin Assembly, and Messenger RNP Complex Biogenesis
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Nap1 Links Transcription Elongation, Chromatin Assembly, and Messenger RNP Complex Biogenesis

机译：Nap1链接转录延伸，染色质大会和信使RNP复杂生物发生。

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Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conserved Saccharomyces cerevisiae histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription of PHO5, and the increase in transcript level and the higher phosphatase activity plateau observed for Δnap1 cells suggest that the net function of Nap1 is to facilitate nucleosome reassembly during transcription elongation. To further our understanding of histone chaperones in transcription elongation, we identified factors that regulate the function of Nap1 in this process. One factor investigated is an essential mRNA export and TREX complex component, Yra1. Nap1 interacts directly with Yra1 and genetically with other TREX complex components and the mRNA export factor Mex67. Additionally, we show that the recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent and that its recruitment to promoters is TREX complex independent. These observations suggest that Nap1 functions provide a new connection between transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.
机译：染色质重塑对于转录延伸的调控至关重要。我们证明了保守的酿酒酵母组蛋白伴侣Nap1与染色质相关。我们发现Nap1调节 PHO5 的转录，并且在Δ nap1 细胞中观察到转录水平的增加和较高的磷酸酶活性平台表明Nap1的净功能是促进转录延伸过程中核小体的重组。为了进一步了解组蛋白伴侣在转录延伸中的作用，我们鉴定了在此过程中调节Nap1功能的因子。研究的一个因素是必需的mRNA输出和TREX复杂成分Yra1。 Nap1直接与Yra1相互作用，并与其他TREX复杂成分和mRNA输出因子Mex67发生遗传相互作用。此外，我们显示Nap1募集到主动转录的基因的编码区域是Yra1依赖的，其募集到启动子是TREX复杂无关的。这些观察结果表明，Nap1功能在转录延伸，染色质组装和信使RNP复合物生物发生之间提供了新的联系。

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