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首页> 外文期刊>Molecular and Cellular Biology >Regulation of the Aurora B Chromosome Passenger Protein Complex during Oocyte Maturation in Xenopus laevis
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Regulation of the Aurora B Chromosome Passenger Protein Complex during Oocyte Maturation in Xenopus laevis

机译：非洲爪蟾卵母细胞成熟过程中的极光B染色体客运蛋白复合体的调节。

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摘要

The dynamics of the Aurora B protein kinase during Xenopus oocyte meiotic maturation were examined. Resting G2 oocytes express inactive Aurora B that is not associated with other subunits of the chromosome passenger complex (CPC). Activity increases near the time of germinal vesicle breakdown in progesterone-treated oocytes, and this increase is correlated with the synthesis of inner centromere protein (INCENP) and survivin, components of the CPC. Ablation of INCENP synthesis led to the failure of progesterone treatment to activate Aurora B, but biochemical progression through the meiosis I-to-II transition and arrest at metaphase II were not affected. At fertilization, Aurora B was deactivated in concert with the degradation of INCENP, and the levels of Aurora B kinase activity and INCENP oscillated in subsequent embryonic cell cycles. Prevention of the decrease in Aurora B activity at fertilization by expression of ectopic wild-type INCENP, but not kinase-dead Aurora B INCENP, blocked calcium-induced exit from metaphase arrest in egg extracts.
机译：研究了非洲爪蟾卵母细胞减数分裂成熟过程中Aurora B蛋白激酶的动力学。静止的G 2 卵母细胞表达无活性的Aurora B，它与染色体乘客复合体（CPC）的其他亚基无关。在孕酮处理的卵母细胞中，在胚泡破裂时，活性增加，并且这种增加与CPC的内部着丝粒蛋白（INCENP）和survivin的合成有关。 INCENP合成的消融导致孕酮治疗无法激活Aurora B，但是通过减数分裂I到II过渡和在中期II停滞的生化进程并未受到影响。受精时，Aurora B随INCENP的降解而失活，并且Aurora B激酶活性和INCENP的水平在随后的胚胎细胞周期中振荡。通过异位野生型INCENP的表达来防止受精时Aurora B活性的降低，而不是通过激酶死亡的Aurora B INCENP的表达，阻止钙诱导的卵提取物中中期停滞的退出。

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