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首页> 外文期刊>Molecular and Cellular Biology >Removal of Frameshift Intermediates by Mismatch Repair Proteins in Saccharomyces cerevisiae
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Removal of Frameshift Intermediates by Mismatch Repair Proteins in Saccharomyces cerevisiae

机译：错配修复蛋白在酿酒酵母中的移码中间体的去除

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Frameshift mutations occur when the coding region of a gene is altered by addition or deletion of a number of base pairs that is not a multiple of three. The occurrence of a deletion versus an insertion type of frameshift depends on the nature of the transient intermediate structure formed during DNA synthesis. Extrahelical bases on the template strand give rise to deletions, whereas extrahelical bases on the strand being synthesized produce insertions. We previously used reversion of a +1 frameshift mutation to analyze the role of the mismatch repair (MMR) machinery in correcting ?1 frameshift intermediates within a defined region of the yeast LYS2gene. In this study, we have used reversion of a ?1 frameshift mutation within the same region of LYS2 to analyze the role of the MMR machinery in the correction of frameshift intermediates that give rise to insertion events. We found that insertion and deletion events occur at similar rates but that the reversion spectra are very different in both the wild-type and MMR-defective backgrounds. In addition, analysis of the +1 spectra revealed novel roles for Msh3p and Msh6p in removing specific types of frameshift intermediates.
机译：当基因的编码区通过添加或删除多个碱基对（不是三个的倍数）而改变时，会发生移码突变。缺失相对于移码的插入类型的发生取决于DNA合成过程中形成的瞬时中间结构的性质。模板链上的螺旋外碱基引起缺失，而合成链上的螺旋外碱基产生插入。我们先前使用+1移码突变的回复来分析错配修复（MMR）机制在纠正酵母 LYS2 基因定义区域内的？1移码中间产物中的作用。在这项研究中，我们使用了在 LYS2 相同区域内的？1移码突变的回复，来分析MMR机制在校正引起插入事件的移码中间体中的作用。我们发现插入和删除事件以相似的速率发生，但是在野生型和MMR缺陷背景下，回复谱都非常不同。此外，对+1光谱的分析揭示了Msh3p和Msh6p在去除特定类型移码中间体中的新作用。

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