...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >The G1 Cyclin Cln3 Promotes Cell Cycle Entry via the Transcription Factor Swi6
【24h】

The G1 Cyclin Cln3 Promotes Cell Cycle Entry via the Transcription Factor Swi6

机译：G1细胞周期蛋白Cln3通过转录因子Swi6促进细胞周期进入

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Saccharomyces cerevisiae (budding yeast), commitment to cell division in late G1 is promoted by the G1 cyclin Cln3 and its associated cyclin-dependent kinase, Cdc28. We show here that all known aspects of the function of Cln3 in G1 phase, including control of cell size, pheromone sensitivity, cell cycle progress, and transcription, require the protein Swi6. Swi6 is a component of two related transcription factors, SBF and MBF, which are known to regulate many genes at the G1-S transition. The Cln3-Cdc28 complex somehow activates SBF and MBF, but there was no evidence for direct phosphorylation of SBF/MBF by Cln3-Cdc28 or for a stable complex between SBF/MBF and Cln3-Cdc28. The activation also does not depend on the ability of Cln3 to activate transcription when artificially recruited directly to a promoter. The amino terminus and the leucine zipper of Swi6 are important for the ability of Swi6 to respond to Cln3 but are not essential for the basal transcriptional activity of Swi6. Cln3-Cdc28 may activate SBF and MBF indirectly, perhaps by phosphorylating some intermediary protein.
机译：在 Saccharomyces cerevisiae （芽芽酵母）中，G 1 细胞周期蛋白Cln3及其相关的细胞周期蛋白-促进了G 1 晚期细胞分裂的决心。依赖性激酶，Cdc28。我们在这里显示，Cln3在G 1 期的功能的所有已知方面，包括对细胞大小，信息素敏感性，细胞周期进程和转录的控制，都需要蛋白质Swi6。 Swi6是两个相关转录因子SBF和MBF的组成部分，已知它们在G 1 -S转换时调控许多基因。 Cln3-Cdc28复合物以某种方式激活了SBF和MBF，但没有证据表明Cln3-Cdc28直接将SBF / MBF磷酸化，也没有证据表明SBF / MBF和Cln3-Cdc28之间存在稳定的复合物。当人为直接募集到启动子时，激活还不取决于Cln3激活转录的能力。 Swi6的氨基末端和亮氨酸拉链对Swi6响应Cln3的能力很重要，但对Swi6的基础转录活性不是必需的。 Cln3-Cdc28可能间接使SBF和MBF活化，可能是通过使某些中间蛋白质磷酸化而实现的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号