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首页> 外文期刊>Molecular and Cellular Biology >UBF Binding In Vivo Is Not Restricted to Regulatory Sequences within the Vertebrate Ribosomal DNA Repeat
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UBF Binding In Vivo Is Not Restricted to Regulatory Sequences within the Vertebrate Ribosomal DNA Repeat

机译：UBF体内结合不限于脊椎动物核糖体DNA重复序列内的调控序列。

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The HMG box containing protein UBF binds to the promoter of vertebrate ribosomal repeats and is required for their transcription by RNA polymerase I in vitro. UBF can also bind in vitro to a variety of sequences found across the intergenic spacer in Xenopus and mammalian ribosomal DNA (rDNA) repeats. The high abundance of UBF, its colocalization with rDNA in vivo, and its DNA binding characteristics, suggest that it plays a more generalized structural role over the rDNA repeat. Until now this view has not been supported by any in vivo data. Here, we utilize chromatin immunoprecipitation from a highly enriched nucleolar chromatin fraction to show for the first time that UBF binding in vivo is not restricted to known regulatory sequences but extends across the entire intergenic spacer and transcribed region of Xenopus, human, and mouse rDNA repeats. These results are consistent with a structural role for UBF at active nucleolar organizer regions in addition to its recognized role in stable transcription complex formation at the promoter.
机译：含有蛋白UBF的HMG盒与脊椎动物核糖体重复序列的启动子结合，是体外RNA聚合酶I转录所需的。 UBF还可以在体外与非洲爪蟾和哺乳动物核糖体DNA（rDNA）重复序列中跨基因间隔区的各种序列结合。 UBF的高丰度，其在体内与rDNA的共定位以及其DNA结合特性表明，它在rDNA重复序列上起着更为普遍的结构作用。到现在为止，这种观点还没有得到任何体内数据的支持。在这里，我们利用高度富集的核仁染色质部分的染色质免疫沉淀来首次证明，UBF体内结合并不局限于已知的调控序列，而是延伸到整个非洲爪蟾的基因间隔区和转录区。 ，人类和小鼠的rDNA重复序列。这些结果除了其在启动子处稳定转录复合物形成中的公认作用外，还与UBF在活性核仁组织区的结构作用一致。

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