Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex

Pei-Yun Jenny Wu; Fred Winston

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Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex

机译：酿酒酵母SAGA共激活复合物中Spt7功能的分析

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The Saccharomyces cerevisiae SAGA complex is required for the normal transcription of a large number of genes. Complex integrity depends on three core subunits, Spt7, Spt20, and Ada1. We have investigated the role of Spt7 in the assembly and function of SAGA. Our results show that Spt7 is important in controlling the levels of the other core subunits and therefore of SAGA. In addition, partial SAGA complexes containing Spt7 can be assembled in the absence of both Spt20 and Ada1. Through biochemical and genetic analyses of a series of spt7 deletion mutants, we have identified a region of Spt7 required for interaction with the SAGA component Spt8. An adjacent Spt7 domain was found to be required for a processed form of Spt7 that is present in a previously identified altered form of SAGA called SLIK, SAGA_alt, or SALSA. Analysis of an spt7 mutant with greatly reduced levels of SLIK/SAGA_alt/SALSA suggests a subtle role for this complex in transcription that may be redundant with a subset of SAGA functions.

机译：酿酒酵母 SAGA复合物是正常转录大量基因所必需的。复杂的完整性取决于三个核心子单元，Spt7，Spt20和Ada1。我们已经研究了Spt7在SAGA的组装和功能中的作用。我们的结果表明，Spt7在控制其他核心亚基以及SAGA的水平中很重要。此外，可以在没有Spt20和Ada1的情况下组装含有Spt7的部分SAGA复合物。通过对一系列 spt7 缺失突变体的生化和遗传分析，我们确定了与SAGA组件Spt8相互作用所需的Spt7区域。发现以先前确定的SAGA变异形式（称为SLIK，SAGA _{alt 或SALSA）存在的Spt7加工形式需要相邻的Spt7域。对SLik / SAGA _{alt / SALSA水平大大降低的 spt7 突变体的分析表明，该复合体在转录中具有微妙的作用，可能与SAGA功能的一部分是多余的。}} 展开▼

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《Molecular and Cellular Biology》 |2002年第15期|共13页

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Pei-Yun Jenny Wu; Fred Winston;
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中图分类细胞生物学;

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专利

1. The Saccharomyces cerevisiae Srb8-Srb11 Complex Functions with the SAGA Complex during Gal4-Activated Transcription [J] . Erica Larschan, Fred Winston Molecular and Cellular Biology . 2005,第1期

机译：酿酒酵母Srb8 Srb11复杂功能与Gal4激活转录过程中的SAGA复杂。

2. Essential Functional Interactions of SAGA, a Saccharomyces cerevisiae Complex of Spt, Ada, and Gcn5 Proteins, With the Snf/Swi and Srb/Mediator Complexes [J] . Fred Winston, Shannon M. Roberts Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1997,第2期

机译：SAGA，Spt，Ada和Gcn5蛋白的酿酒酵母复合物与Snf / Swi和Srb /介体复合物的基本功能相互作用

3. C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complex [J] . Stephen MT Hoke, Gaoyang Liang, A Irina Mutiu BMC Biochemistry . 2007,第16期

机译：在没有功能性SAGA复合物的情况下发生酵母Spt7的C末端加工

4. Quantitative proteomic analysis reveals environmental interaction and epistasis in the responses to complex stimuli in Saccharomyces cerevisiae. [C] . Parimal Samir, Rahul, James C. Slaughter, ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：定量蛋白质组学分析揭示了对酿酒酵母酿酒酵母复杂刺激的反应中的环境相互作用和外观。

5. Functional and structural analysis of the Saccharomyces cerevisiae SAGA coactivator complex. [D] . Wu, Pei-Yun Jenny. 2004

机译：酿酒酵母SAGA共激活物复合物的功能和结构分析。

6. Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex [O] . Pei-Yun Jenny Wu, Fred Winston 2002

机译：酿酒酵母SAGA共激活复合物中Spt7功能的分析

7. Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex [O] . Wu, Pei-Yun Jenny, Winston, Fred 2002

机译：酿酒酵母SAGA共激活复合物中Spt7功能的分析

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Analysis of Spt7 Function in the Saccharomyces cerevisiae SAGA Coactivator Complex

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