• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Spliceosome Assembly Pathways for Different Types of Alternative Splicing Converge during Commitment to Splice Site Pairing in the A Complex
【24h】

Spliceosome Assembly Pathways for Different Types of Alternative Splicing Converge during Commitment to Splice Site Pairing in the A Complex

机译：不同类型的可变剪接的熔接体组装途径在致力于复合体中的剪接位点配对过程中会聚。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Differential splice site pairing establishes alternative splicing patterns resulting in the generation of multiple mRNA isoforms. This process is carried out by the spliceosome, which is activated by a series of sequential structural rearrangements of its five core snRNPs. To determine when splice sites become functionally paired, we carried out a series of kinetic trap experiments using pre-mRNAs that undergo alternative 5′ splice site selection or alternative exon inclusion. We show that commitment to splice site pairing in both cases occurs in the A complex, which is characterized by the ATP-dependent association of the U2 snRNP with the branch point. Interestingly, the timing of splice site pairing is independent of the intron or exon definition modes of splice site recognition. Using the ATP analog ATPγS, we showed that ATP hydrolysis is required for splice site pairing independent from U2 snRNP binding to the pre-mRNA. These results identify the A complex as the spliceosomal assembly step dedicated to splice site pairing and suggest that ATP hydrolysis locks splice sites into a splicing pattern after stable U2 snRNP association to the branch point.
机译：差异剪接位点配对建立了可替代的剪接模式，从而导致产生了多个mRNA同工型。该过程由剪接体进行，该剪接体由其五个核心snRNP的一系列顺序结构重排激活。为了确定剪接位点何时在功能上配对，我们使用了进行替代5'剪接位点选择或替代外显子包涵的pre-mRNA，进行了一系列动力学陷阱实验。我们表明，在两种情况下，对剪接位点配对的承诺均发生在A复合物中，其特征在于U2 snRNP与分支点的ATP依赖关系。有趣的是，剪接位点配对的时间与剪接位点识别的内含子或外显子定义模式无关。使用ATP类似物ATPγS，我们证明了ATP水解是拼接位点配对所必需的，而该配对位点与U2 snRNP结合前mRNA无关。这些结果将A复合物鉴定为专门用于剪接位点配对的剪接体组装步骤，并表明ATP水解在稳定的U2 snRNP结合至分支点后将剪接位点锁定为剪接模式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号