Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box.

J M Li; R A Parsons; W F Marzluff

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Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box.

机译：在体外转录海胆U6基因需要一个TATA样盒，一个近端序列元件和一个与海胆USF结合的E盒。

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The tandemly repeated gene set encoding the sea urchin U6 gene has been cloned from the sea urchin Strongylocentrotus purpuratus. The U6 gene is transcribed by RNA polymerase III in a sea urchin nuclear extract. Like that of the vertebrate U6 genes, transcription of the sea urchin U6 gene does not require any internal sequences or 3' sequences but requires only 5' flanking sequences. Only 88 nucleotides of 5' flanking sequence are required for maximal expression in vitro. Mutagenesis experiments demonstrated the requirement for three elements, a CACGTG element at -80, a proximal sequence element at about -55, and the TATA-like box at -25. The major protein in sea urchin extracts that interacts with the CACGTG element is sea urchin USF, and immunodepletion of sea urchin USF greatly reduces transcription. The USF binding site in the U6 gene is highly homologous (11 of 13 nucleotides) with the USF binding sites found in the promoter of the S. purpuratus spec genes.

机译：从海胆Strongylocentrotus purpuratusus中克隆了编码海胆U6基因的串联重复基因集。 U6基因由RNA聚合酶III在海胆核提取物中转录。与脊椎动物U6基因的转录类似，海胆U6基因的转录不需要任何内部序列或3'序列，而仅需要5'侧翼序列。 5'侧翼序列的88个核苷酸是体外最大表达所需要的。诱变实验表明需要三个元素，CACGTG元素为-80，近端序列元素为约-55，TATA样盒为-25。与CACGTG元件相互作用的海胆提取物中的主要蛋白质是海胆USF，海胆USF的免疫耗竭大大降低了转录。 U6基因中的USF结合位点与紫癜谱基因启动子中的USF结合位点高度同源（13个核苷酸中的11个核苷酸）。

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《Molecular and Cellular Biology》 |1994年第3期|共10页
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J M Li; R A Parsons; W F Marzluff;
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中图分类细胞生物学;
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1. Common factors direct transcription through the proximal sequence elements (PSEs) of the embryonic sea urchin U1, U2, and U6 genes despite minimal similarity among the PSEs. [J] . J M Li, R P Haberman, W F Marzluff Molecular and Cellular Biology . 1996,第3期

机译：尽管PSE之间的相似性极小，但公共因素仍通过胚胎海胆U1，U2和U6基因的近端序列元件（PSE）指导转录。
2. Characterization of two developmentally regulated sea urchin U2 small nuclear RNA promoters: a common required TATA sequence and independent proximal and distal elements. [J] . B Stefanovic, W F Marzluff Molecular and Cellular Biology . 1992,第2期

机译：表征两个受发育调节的海胆U2小核RNA启动子：共同需要的TATA序列和独立的近端和远端元件。
3. Repetitive DNA sequences linked to the sea urchin spec genes contain transcriptional enhancer-like elements☆ [J] . Lin Gan, Wei Zhang, William H. Klein Developmental biology . 1990,第1期

机译：与海胆规格基因相关的重复DNA序列含有转录增强子样元件☆
4. Expression of Pigment Cell-Specific Genes in the Ontogenesis of the Sea Urchin Strongylocentrotus intermedius [C] . Natalya V. Ageenko, Konstantin V. Kiselev, Nelly A. Odintsova International Marine Biotechnology Conference . 2010

机译：色素细胞特异性基因在中间型海胆本体发育中的表达
5. The T-box family of transcription factors in sea urchin development: Involvement in pattern formation and morphogenesis. [D] . Gross, Jeffrey Martin. 2002

机译：海胆发育中转录因子的T-box家族：参与模式形成和形态发生。
6. Transcription of the sea urchin U6 gene in vitro requires a TATA-like box a proximal sequence element and sea urchin USF which binds an essential E box. [O] . J M Li, R A Parsons, W F Marzluff 1994

机译：要在体外转录海胆U6基因需要一个TATA样盒一个近端序列元件和一个海胆USF后者必须结合一个必需的E盒。
7. Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box. [O] . Li, J M, Parsons, R A, Marzluff, W F 1994

机译：要在体外转录海胆U6基因，需要一个TATA样盒，一个近端序列元件和一个海胆USF，后者必须结合一个必需的E盒。

Transcription of the sea urchin U6 gene in vitro requires a TATA-like box, a proximal sequence element, and sea urchin USF, which binds an essential E box.

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