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首页> 外文期刊>Molecular and Cellular Biology >Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint.
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Single-stranded DNA arising at telomeres in cdc13 mutants may constitute a specific signal for the RAD9 checkpoint.

机译：在cdc13突变体的端粒处产生的单链DNA可能构成RAD9检查点的特定信号。

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摘要

A cdc13 temperature-sensitive mutant of Saccharomyces cerevisiae arrests in the G2 phase of the cell cycle at the restrictive temperature as a result of DNA damage that activates the RAD9 checkpoint. The DNA lesions present after a failure of Cdc13p function appear to be located almost exclusively in telomere-proximal regions, on the basis of the profile of induced mitotic recombination. cdc13 rad9 cells dividing at the restrictive temperature contain single-stranded DNA corresponding to telomeric and telomere-proximal DNA sequences and eventually lose telomere-associated sequences. These results suggest that the CDC13 product functions in telomere metabolism, either in the replication of telomeric DNA or in protecting telomeres from the double-strand break repair system. Moreover, since cdc13 rad9 cells divide at a wild-type rate for several divisions at the restrictive temperature while cdc13 RAD9 cells arrest in G2, these results also suggest that single-stranded DNA may be a specific signal for the RAD9 checkpoint.
机译：由于激活RAD9检查点的DNA损伤，啤酒酵母的cdc13温度敏感突变体在限制性温度下在细胞周期的G2期停滞。根据诱导的有丝分裂重组情况，Cdc13p功能衰竭后出现的DNA损伤似乎几乎完全位于端粒附近。在限制温度下分裂的cdc13 rad9细胞包含对应于端粒和端粒近端DNA序列的单链DNA，最终丢失了端粒相关的序列。这些结果表明，CDC13产物在端粒代谢中起作用，在端粒DNA的复制中或在保护端粒免受双链断裂修复系统的作用中。此外，由于cdc13 rad9细胞在限制温度下以野生型速率分裂数次，而cdc13 RAD9细胞则停滞在G2中，因此这些结果还表明，单链DNA可能是RAD9检查点的特定信号。

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