• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3.
【24h】

A region rich in aspartic acid, arginine, tyrosine, and glycine (DRYG) mediates eukaryotic initiation factor 4B (eIF4B) self-association and interaction with eIF3.

机译：富含天冬氨酸，精氨酸，酪氨酸和甘氨酸（DRYG）的区域介导了真核起始因子4B（eIF4B）的自缔合和与eIF3的相互作用。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The binding of mRNA to the ribosome is mediated by eukaryotic initiation factors eukaryotic initiation factor 4F (eIF4F), eIF4B, eIF4A, and eIF3, eIF4F binds to the mRNA cap structure and, in combination with eIF4B, is believed to unwind the secondary structure in the 5' untranslated region to facilitate ribosome binding. eIF3 associates with the 40S ribosomal subunit prior to mRNA binding. eIF4B copurifies with eIF3 and eIF4F through several purification steps, suggesting the involvement of a multisubunit complex during translation initiation. To understand the mechanism by which eIF4B promotes 40S ribosome binding to the mRNA, we studied its interactions with partner proteins by using a filter overlay (protein-protein [far Western]) assay and the two-hybrid system. In this report, we show that eIF4B self-associates and also interacts directly with the p170 subunit of eIF3. A region rich in aspartic acid, arginine, tyrosine, and glycine, termed the DRYG domain, is sufficient for self-association of eIF4B, both in vitro and in vivo, and for interaction with the p170 subunit of eIF3. These experiments suggest that eIF4B participates in mRNA-ribosome binding by acting as an intermediary between the mRNA and eIF3, via a direct interaction with the p170 subunit of eIF3.
机译：mRNA与核糖体的结合是由真核起始因子4F（eIF4F），eIF4B，eIF4A和eIF3介导的，eIF4F与mRNA帽结构结合，并与eIF4B结合，可以解开5'非翻译区促进核糖体结合。 eIF3在mRNA结合之前与40S核糖体亚基缔合。 eIF4B通过几个纯化步骤与eIF3和eIF4F共纯化，表明翻译起始过程中涉及了一个多亚基复合物。为了了解eIF4B促进40S核糖体与mRNA结合的机制，我们通过使用滤膜覆盖（蛋白质-蛋白质[far Western]）测定法和双杂交系统研究了它与伴侣蛋白质的相互作用。在此报告中，我们显示eIF4B自相关并且也直接与eIF3的p170亚基相互作用。被称为DRYG结构域的富含天冬氨酸，精氨酸，酪氨酸和甘氨酸的区域足以在体外和体内自缔合eIF4B，并足以与eIF3的p170亚基相互作用。这些实验表明，eIF4B通过与eIF3的p170亚基直接相互作用，通过充当mRNA与eIF3之间的中介而参与了mRNA-核糖体的结合。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号