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首页> 外文期刊>Molecular and Cellular Biology >Formation of extrachromosomal circular amplicons with direct or inverted duplications in drug-resistant Leishmania tarentolae.
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Formation of extrachromosomal circular amplicons with direct or inverted duplications in drug-resistant Leishmania tarentolae.

机译：在耐药性塔氏弧菌中直接或反向重复的染色体外环状扩增子的形成。

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摘要

Selection for methotrexate resistance in Leishmania spp. is often associated with amplification of the H locus short-chain dehydrogenase-reductase gene ptr1 as part of extrachromosomal elements. Extensive sequences are always coamplified and often contain inverted duplications, most likely formed by the annealing of inverted repeats present at the H locus. By gene targeting mediated by homologous recombination, several repeated sequences were introduced in the vicinity of ptr1. Selection for methotrexate resistance in these transfectants led to ptr1 amplification as part of small circles with direct or inverted duplications whether the integrated sequences consisted of direct or inverted repeats. Hence, for a region to he amplified in L. tarentolae during drug selection, a drug resistance gene is required and must be flanked by (any) homologous repeated sequences. The distance between these repeats and their orientation will determine the length of the amplicon and whether it contains direct or inverted duplications.
机译：利什曼原虫（Leishmania spp）对氨甲蝶呤抗性的选择。通常与H位点短链脱氢酶还原酶基因ptr1的扩增有关，这是染色体外元件的一部分。广泛的序列总是被共扩增，并且通常包含反向重复，这很可能是由H基因座上反向重复序列的退火所形成的。通过同源重组介导的基因靶向，在ptr1附近引入了几个重复序列。这些转染子中甲氨蝶呤抗性的选择导致ptr1扩增为小圆圈的一部分，具有直接或反向重复，无论整合序列是由直接或反向重复组成。因此，对于在药物选择过程中他在塔伦支乳杆菌中扩增的区域，需要药物抗性基因，并且该基因必须侧翼为（任何）同源重复序列。这些重复之间的距离及其方向将决定扩增子的长度，以及它是否包含直接或反向重复。

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