...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae.
【24h】

A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae.

机译：包含两个转录因子的复合物调节酿酒酵母中的过氧化物酶体增殖和β-氧化酶的协同诱导。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Expression of the POX1 gene, which encodes peroxisomal acyl coenzyme A oxidase in the yeast Saccharomyces cerevisiae, is tightly regulated and can be induced by fatty acids such as oleate. Previously we have shown that this regulation is brought about by interactions between trans-acting factor(s) and an upstream activating sequence (UAS1) in the POX1 promoter. We recently identified and isolated a transcription factor, Oaf1p, that binds to the UAS1 of POX1 and mediates its induction. A screening strategy has been developed and used to identify eight S. cerevisiae mutants, from three complementation groups, that are defective in the oleate induction of POX1. Characterization of one such mutant led to the identification of Oaf2p, a protein that is 39% identical to Oaf1p. Oaf1p and Oaf2p form a protein complex that is required for the activation of POX1 and FOX3 and for proliferation of peroxisomes. We propose a model in which these two transcription factors heterodimerize and mediate this activation process. The mutants that we have isolated, and further identification of the corresponding defective genes, provide us with an opportunity to characterize the mechanisms involved in the coordinate regulation of peroxisomal beta-oxidation enzymes.
机译：酵母酿酒酵母中编码过氧化物酶体酰基辅酶A氧化酶的POX1基因的表达受到严格调节，并可由脂肪酸（如油酸盐）诱导。先前我们已经表明，该调节是由反式作用因子与POX1启动子中的上游激活序列（UAS1）之间的相互作用引起的。我们最近鉴定并分离出了转录因子Oaf1p，它与POX1的UAS1结合并介导其诱导。已经开发了一种筛选策略，用于从三个互补组中鉴定出在油酸诱导POX1中有缺陷的八个酿酒酵母突变体。一种这样的突变体的表征导致鉴定了Oaf2p，该蛋白与Oaf1p的同源性为39％。 Oaf1p和Oaf2p形成激活POX1和FOX3以及过氧化物酶体增殖所需的蛋白质复合物。我们提出了一个模型，其中这两个转录因子异二聚体并介导此激活过程。我们已经分离出的突变体，并进一步鉴定出相应的缺陷基因，为我们提供了表征过氧化物酶体β-氧化酶的协调调控机制的机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号