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首页> 外文期刊>Molecular and Cellular Biology >A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase.
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A family of cyclin-like proteins that interact with the Pho85 cyclin-dependent kinase.

机译：与Pho85细胞周期蛋白依赖性激酶相互作用的细胞周期蛋白样蛋白家族。

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In budding yeast, entry into the mitotic cell cycle, or Start, requires the Cdc28 cyclin-dependent kinase (Cdk) and one of its three associated G1 cyclins, Cln1, Cln2, or Cln3. In addition, two other G1 cyclins, Pcl1 and Pcl2, associate with a second Cdk, Pho85, to contribute to Start. Although Pho85 is not essential for viability, Pcl1,2-Pho85 kinase complexes become essential for Start in the absence of Cln1,2-Cdc28 kinases. In addition, Pho85 interacts with a third cyclin, Pho80, to regulate acid phosphatase gene expression. Other cellular roles for Pho85 cyclin-Cdk complexes are suggested by the multiple phenotypes associated with deletion of PHO85, in addition to Start defects and deregulated acid phosphatase gene expression. Strains with pho80, pcl1, and pcl2 deletions show only a subset of the pho85 mutant phenotypes, suggesting the existence of additional Pho85 cyclins (Pcls). We used two-hybrid screening and database searching to identify seven additional cyclin-related genes that may interact with Pho85. We found that all of the new genes encode proteins that interacted with Pho85 in an affinity chromatography assay. One of these genes, CLG1, was previously suggested to encode a cyclin, based on the protein's sequence homology to Pcl1 and Pcl2. We have named the other genes PCL5, PCL6, PCL7, PCL8, PCL9, and PCL10. On the basis of sequence similarities, the PCLs can be divided into two subfamilies: the Pcl1,2-like subfamily and the Pho80-like subfamily. We found that deletion of members of the Pcl1,2 class of genes resulted in pronounced morphological abnormalities. In addition, we found that expression of one member of the Pcl1,2 subfamily, PCL9, is cell cycle regulated and is decreased in cells arrested in G1 by pheromone treatment. Our studies suggest that Pho85 associates with multiple cyclins and that subsets of cyclins may direct Pho85 to perform distinct roles in cell growth and division.
机译：在发芽酵母中，进入有丝分裂细胞周期或开始需要Cdc28细胞周期蛋白依赖性激酶（Cdk）及其三个相关的G1细胞周期蛋白之一Cln1，Cln2或Cln3。此外，另外两个G1细胞周期蛋白Pcl1和Pcl2与第二个Cdk Pho85相关联，以促进启动。尽管Pho85对生存力不是必需的，但是在不存在Cln1,2-Cdc28激酶的情况下，Pcl1,2-Pho85激酶复合物对于Start而言是必不可少的。此外，Pho85与第三个细胞周期蛋白Pho80相互作用，以调节酸性磷酸酶基因的表达。除起始缺陷和酸磷酸酶基因表达失调外，与PHO85缺失相关的多种表型还提示了Pho85细胞周期蛋白-Cdk复合物的其他细胞作用。带有pho80，pcl1和pcl2缺失的菌株仅显示pho85突变表型的一个子集，表明存在其他Pho85 cyclins（Pcls）。我们使用了两个杂交筛选和数据库搜索来识别七个其他可能与Pho85相互作用的细胞周期蛋白相关基因。我们发现，所有新基因都编码在亲和色谱分析中与Pho85相互作用的蛋白质。这些蛋白质之一，CLG1，以前曾被建议根据细胞与Pcl1和Pcl2的序列同源性来编码细胞周期蛋白。我们将其他基因命名为PCL5，PCL6，PCL7，PCL8，PCL9和PCL10。根据序列相似性，PCL可以分为两个亚家族：Pcl1,2样亚家族和Pho80样亚家族。我们发现删除Pcl1,2类基因的成员会导致明显的形态异常。此外，我们发现，Pcl1,2亚家族的一个成员PCL9的表达受到细胞周期的调节，并且在通过信息素处理而被捕集到G1的细胞中减少。我们的研究表明，Pho85与多个细胞周期蛋白相关，细胞周期蛋白的子集可能指导Pho85在细胞生长和分裂中发挥不同的作用。

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