...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A Cellular Repressor of E1A-Stimulated Genes That Inhibits Activation by E2F
【24h】

A Cellular Repressor of E1A-Stimulated Genes That Inhibits Activation by E2F

机译：E1A刺激基因抑制E2F激活的细胞阻遏物。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. Here we report the identification and characterization of a cellular protein that antagonizes transcriptional activation and cellular transformation by E1A. This protein, termed CREG for cellular repressor of E1A-stimulated genes, shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. In transfection assays, CREG represses transcription and antagonizes 12SE1A-mediated activation of both the adenovirus E2 and cellular hsp70 promoters. CREG also antagonizes E1A-mediated transformation, as expression of CREG reduces the efficiency with which E1A and the oncogeneras cooperate to transform primary cells. Binding sites for E2F, a key transcriptional regulator of cell cycle progression, were found to be required for repression of the adenovirus E2 promoter by CREG, and CREG was shown to inhibit activation by E2F. Since both the adenovirus E1A protein and transcriptional activation by E2F function to promote cellular proliferation, the results presented here suggest that CREG activity may contribute to the transcriptional control of cell growth and differentiation.
机译：腺病毒E1A蛋白既激活又抑制基因表达，从而促进细胞增殖并抑制分化。在这里，我们报告的拮抗转录激活和E1A细胞转化的细胞蛋白质的鉴定和表征。这种蛋白质被称为CREG，用于E1A刺激的基因的细胞阻遏物，与E1A具有有限的序列相似性，并且在体外与普通转录因子TBP和肿瘤抑制物pRb结合。在转染测定中，CREG抑制腺病毒E2和细胞hsp70启动子的转录并拮抗12SE1A介导的激活。 CREG还拮抗E1A介导的转化，因为CREG的表达降低了E1A和癌基因 ras 协同转化原代细胞的效率。发现E2F的结合位点是细胞周期进程的关键转录调节因子，被CREG抑制腺病毒E2启动子是必需的，而且CREG被证明可以抑制E2F的激活。由于腺病毒E1A蛋白和E2F的转录激活均具有促进细胞增殖的功能，因此此处显示的结果表明CREG活性可能有助于细胞生长和分化的转录控制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号