The 3′→5′ Exonucleases of DNA Polymerases δ and ? and the 5′→3′ Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae

Hiep T. Tran; Dmitry A. Gordenin; Michael A. Resnick

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The 3′→5′ Exonucleases of DNA Polymerases δ and ? and the 5′→3′ Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae

机译：DNA聚合酶δ和δ的3'→5'核酸外切酶和5'→3'核酸外切酶Exo1在酿酒酵母中避免复制后突变中起主要作用

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Replication fidelity is controlled by DNA polymerase proofreading and postreplication mismatch repair. We have genetically characterized the roles of the 5′→3′ Exo1 and the 3′→5′ DNA polymerase exonucleases in mismatch repair in the yeast Saccharomyces cerevisiae by using various genetic backgrounds and highly sensitive mutation detection systems that are based on long and short homonucleotide runs. Genetic interactions were examined among DNA polymerase ? (pol2-4) and δ (pol3-01) mutants defective in 3′→5′ proofreading exonuclease, mutants defective in the 5′→3′ exonuclease Exo1, and mismatch repair mutants (msh2, msh3, or msh6). These three exonucleases play an important role in mutation avoidance. Surprisingly, the mutation rate in an exo1 pol3-01 mutant was comparable to that in an msh2 pol3-01 mutant, suggesting that they participate directly in postreplication mismatch repair as well as in other DNA metabolic processes.

机译：复制保真度受DNA聚合酶校对和复制后错配修复的控制。我们已经通过各种遗传背景和高度敏感的突变检测系统对5'→3'Exo1和3'→5'DNA聚合酶核酸外切酶在酵母错配修复中的作用进行了遗传学表征。基于长和短的同核苷酸序列。检查DNA聚合酶之间的遗传相互作用？（ pol2-4 ）和δ（ pol3-01 ）突变体在3'→5'校对核酸外切酶中有缺陷，突变体在5'→3'核酸外切酶Exo1中有缺陷，以及错配修复突变体（ msh2 ， msh3 或 msh6 ）。这三个外切核酸酶在避免突变中起重要作用。令人惊讶的是， exo1 pol3-01 突变体的突变率与 msh2 pol3-01 突变体的突变率相当，这表明它们直接参与复制后错配修复以及在其他DNA代谢过程中。 展开▼

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《Molecular and Cellular Biology》 |1999年第3期|共8页

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Hiep T. Tran; Dmitry A. Gordenin; Michael A. Resnick;
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中图分类细胞生物学;

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1. The 3′→5′ Exonucleases of DNA Polymerases δ and ? and the 5′→3′ Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae [J] . Hiep T. Tran, Dmitry A. Gordenin, Michael A. Resnick Molecular and Cellular Biology . 1999,第3期

机译：DNA聚合酶δ和δ的3'→5'核酸外切酶和5'→3'核酸外切酶Exo1在酿酒酵母中避免复制后突变中起主要作用

2. The Multiple Biological Roles of the 3′→5′ Exonuclease of Saccharomyces cerevisiae DNA Polymerase δ Require Switching between the Polymerase and Exonuclease Domains [J] . Yong Hwan Jin, Parie Garg, Carrie M. W. Stith, Molecular and Cellular Biology . 2005,第1期

机译：酿酒酵母DNA聚合酶δ3'→5'核酸外切酶的多重生物学作用需要在聚合酶和核酸外切酶结构域之间切换

3. 3′ → 5′ Exonucleases of DNA Polymerases ? and δ Correct Base Analog Induced DNA Replication Errors on Opposite DNA Strands in Saccharomyces cerevisiae [J] . Polina V. Shcherbakova, Youri I. Pavlov Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1996,第3期

机译：DNA聚合酶的3'→5'外切核酸酶和δ正确碱基类似物在酿酒酵母中相反的DNA链上引起DNA复制错误

4. HDX/MS of the E. coli replicative DNA polymerase identifies stabilizing interactions between the DNA sliding clamp, exonuclease and clamp loader [C] . Rafael Fernandez Leiro, Sarah L. Maslen, Meindert H. Lamers, ASMS Conference on Mass Spectrometry and Allied Topics . 2016

机译：大肠杆菌复制DNA聚合酶的HDX / MS识别DNA滑动夹，外切核酸酶和夹具装载机之间的稳定相互作用

5. Using Saccharomyces cerevisiae to Study the Role of the X Family DNA Polymerase POL4 in CRISPR/Cas9-Induced Mutation [D] . Zhang, Richard 2018

机译：使用酿酒酵母研究X家族DNA聚合酶POL4在CRISPR / Cas9诱导的突变中的作用

6. The 3′→5′ Exonucleases of DNA Polymerases δ and ɛ and the 5′→3′ Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae [O] . Hiep T. Tran, Dmitry A. Gordenin, Michael A. Resnick 1999

机译：DNA聚合酶δ和3的3→5核酸外切酶和5→3核酸外切酶Exo1在酿酒酵母中避免复制后突变中起主要作用

7. The multiple biological roles of the 3’→5’ exonuclease of Saccharomyces cerevisiae DNA polymerase δ require switching between the polymerase and exonuclease domains [O] . Jin Yong Hawn, Garg Parie, Stith Carrie M. W., 2005

机译：酿酒酵母DNA聚合酶δ的3'→5'核酸外切酶的多种生物学作用需要在聚合酶和核酸外切酶结构域之间切换

1. SPR生物传感器研究缺乏3′→5′核酸外切酶活性的DNA聚合酶对DNA的分子识别功能 [J] . 吴蕾 ,黄明辉 ,赵建龙 . 生物化学与生物物理进展 . 2005,第012期

2. 核酸外切酶Ⅷ截短体的重组表达及其在体外DNA重组反应中的应用 [J] . 朱燕 ,韩小韦 ,牛毅男 . 生物工程学报 . 2019,第005期

3. DNA聚合酶Pol(t)在紫外损伤诱导基因组高突变中的作用 [J] . 曾益军 ,周虎传 ,位全芳 . 医学分子生物学杂志 . 2008,第006期

4. 基于二硫化钨和核酸外切酶Ⅲ的荧光法高灵敏检测血液中汞离子 [J] . 耿琴 . 中国药师 . 2019,第004期

5. 基于核酸外切酶Ⅰ的适配体荧光传感器检测人尿液中的Hg2+ [J] . 赵秋伶 ,张尤华 ,于晓艳 . 分析化学 . 2016,第007期

6. 食管癌中DNA聚合酶β基因突变的研究 [C] . 董子明 ,赵国强 ,赵勤 . 第六届海内外生命科学论坛 . 2001

7. DNA聚合酶κ和DNA聚合酶η在烷化剂MNNG引起的非定标突变中的作用 [A] . 罗月球 . 2003

1. 实时PCR检测中抗高温聚合酶核酸外切酶活性的荧光探针 [P] . 中国专利： CN1900312B . 2016.08.03

2. 实时PCR检测中抗高温聚合酶核酸外切酶活性的荧光探针 [P] . 中国专利： CN1900312A . 2007-01-24

3. 5' TO 3' EXONUCLEASE MUTATIONS OF THERMOSTABLE DNA POLYMERASES [P] . 外国专利： CA2090614C . 2001-12-11

机译：可热DNA聚合酶的5'至3'核酸外切突变

4. 5' TO 3' EXONUCLEASE MUTATIONS OF THERMOSTABLE DNA POLYMERASES [P] . 外国专利： GR3031152T3 . 1999-12-31

机译：可热DNA聚合酶的5'至3'核酸外切突变

5. Mutations in the 5 'to 3' exonuclease of DNA polymerases. [P] . 外国专利： ES2134198T3 . 1999-10-01

机译：DNA聚合酶5'至3'核酸外切酶中的突变。

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The 3′→5′ Exonucleases of DNA Polymerases δ and ? and the 5′→3′ Exonuclease Exo1 Have Major Roles in Postreplication Mutation Avoidance in Saccharomyces cerevisiae

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