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首页> 外文期刊>Molecular and Cellular Biology >A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1.
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A negative regulator of HO transcription, SIN1 (SPT2), is a nonspecific DNA-binding protein related to HMG1.

机译：HO转录的负调控因子SIN1（SPT2）是与HMG1相关的非特异性DNA结合蛋白。

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The SIN1 gene was initially identified because mutations in SIN1 bypass the need for SWI1 to activate transcription of the yeast HO gene. We show here that transcription of HO in swi1 sin1 cells efficiently utilizes the normal start site. We have cloned SIN1 and found that it is identical to the previously identified gene SPT2, mutations in which allow transcription from certain mutated regulatory regions. The predicted SIN1/SPT2 protein has a distinctive amino acid composition (45% charged residues, 25% basic and 20% acidic) and has similarity to the mammalian HMG1 protein, a nonhistone component of chromatin. We show that SIN1 is concentrated in the nucleus and binds to DNA with little or no sequence specificity in vitro. It thus exhibits properties of an HMG protein. Addition of random DNA segments to a test promoter alters regulation by SIN1 in a manner similar to addition of a segment from the HO upstream region. Functional analysis of certain SIN1 mutations suggests that SIN1 may be part of a multiprotein complex. On the basis of these results, we propose that SIN1 is a nonhistone component of chromatin which creates the proper context for transcription. Because sin1 mutants exhibit increased loss of chromosome III, SIN1 may also play a role in fidelity of chromosome segregation.
机译：最初鉴定出SIN1基因是因为SIN1中的突变绕过了SWI1激活酵母HO基因转录的需要。我们在这里显示swi1 sin1细胞中HO的转录有效利用了正常的起始位点。我们已经克隆了SIN1，发现它与先前鉴定的基因SPT2相同，即允许从某些突变的调控区转录的突变。预测的SIN1 / SPT2蛋白具有独特的氨基酸组成（45％带电残基，25％碱性残基和20％酸性），并且与哺乳动物HMG1蛋白（染色质的非组蛋白成分）相似。我们表明，SIN1集中在细胞核中，与DNA结合时几乎没有或没有序列特异性。因此，它表现出HMG蛋白的特性。向测试启动子添加随机DNA片段以类似于从HO上游区域添加片段的方式改变SIN1的调控。某些SIN1突变的功能分析表明SIN1可能是多蛋白复合物的一部分。根据这些结果，我们建议SIN1是染色质的非组蛋白成分，可为转录创造适当的环境。由于sin1突变体表现出增加的第III号染色体丢失，因此SIN1也可能在染色体分离的保真度中起作用。

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