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首页> 外文期刊>Molecular and Cellular Biology >Cellular ras activity is required for passage through multiple points of the G0/G1 phase in BALB/c 3T3 cells.
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Cellular ras activity is required for passage through multiple points of the G0/G1 phase in BALB/c 3T3 cells.

机译：细胞ras活性需要通过BALB / c 3T3细胞中G0 / G1期的多个点。

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Microinjection experiments demonstrated a requirement for cellular ras activity late in G1. In this study, we used two separate methods to identify an additional requirement for cellular ras activity early in the G0/G1 phase of the cell cycle. Quiescent BALB/c cells were injected with anti-ras antibody prior to stimulation with serum. The cells would therefore be inhibited in progression through the cell cycle at the earliest point requiring ras function. Alternatively, cells were inhibited in late G1 as in previous studies by injecting anti-ras several hours after serum addition to quiescent cells. The injected cultures were then treated with chemical cell cycle inhibitors known to function in mid-G1. Cells injected with anti-ras prior to serum stimulation were retained at a point of ras requirement prior to the execution point of the chemical inhibitor, while cells injected 3 to 5 h after serum stimulation were retained at a point of ras requirement downstream of the execution point of the chemical inhibitor. To confirm these results, quiescent BALB/c cells were injected with anti-ras antibody prior to or several hours following serum addition. In this case, however, second injections of oncogenic ras or adenoviral E1A protein were performed to overcome the inhibitory effects of the anti-ras antibody. Cells injected prior to serum addition were clearly inhibited at an early point of Ras requirement since they required 5 or 6 h longer to enter S phase than cells injected with anti-ras antibody after serum addition.
机译：显微注射实验证明在G1晚期需要细胞ras活性。在这项研究中，我们使用了两种单独的方法来确定细胞周期G0 / G1早期对细胞ras活性的其他需求。在用血清刺激之前，向静止的BALB / c细胞注射抗ras抗体。因此，在需要ras功能的最早点，细胞将在整个细胞周期中受到抑制。或者，如先前研究中所述，在血清添加到静止细胞后数小时注射抗ras，从而在G1期晚期抑制细胞。然后用已知在G1中期起作用的化学细胞周期抑制剂处理注入的培养物。血清刺激前注射抗ras的细胞保留在化学抑制剂执行点之前的ras要求点，而血清刺激后3至5小时注射的细胞保留在执行下游的ras要求点。点的化学抑制剂。为了证实这些结果，在加入血清之前或之后数小时向静止的BALB / c细胞注射抗ras抗体。但是，在这种情况下，要进行第二次致癌性ras或腺病毒E1A蛋白注射，以克服抗ras抗体的抑制作用。加入血清之前注射的细胞在Ras需求的早期明显受到抑制，因为与进入血清后注射抗ras抗体的细胞相比，进入S期需要更长的5或6小时。

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