Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1.

E J Steinmetz; D A Brow

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Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1.

机译：通过与异质核核糖核蛋白样蛋白Nrd1和推定解旋酶Sen1协同作用的外源序列元件抑制基因表达。

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We have fortuitously identified a nucleotide sequence that decreases expression of a reporter gene in the yeast Saccharomyces cerevisiae 20-fold when inserted into an intron. The primary effect of the insertion is a decrease in pre-mRNA abundance accompanied by the appearance of 3'-truncated transcripts, consistent with premature transcriptional termination and/or pre-mRNA degradation. Point mutations in the cis element relieve the negative effect, demonstrating its sequence specificity. A novel yeast protein, named Nrd1, and a previously identified putative helicase, Sen1, help mediate the negative effect of the cis element. Sen1 is an essential nuclear protein that has been implicated in a variety of nuclear functions. Nrd1 has hallmarks of a heterogeneous nuclear ribonucleoprotein, including an RNA recognition motif, a region rich in RE and RS dipeptides, and a proline- and glutamine-rich domain. An N-terminal domain of Nrd1 may facilitate direct interaction with RNA polymerase II. Disruption of the NRD1 gene is lethal, yet C-terminal truncations that delete the RNA recognition motif and abrogate the negative effect of the cis element nevertheless support cell growth. Thus, expression of a gene containing the cis element could be regulated through modulation of the activity of Nrd1. The recent identification of Nrd1-related proteins in mammalian cells suggests that this potential regulatory pathway is widespread among eukaryotes.

机译：我们已经幸运地确定了插入到内含子中的一种核苷酸序列，该核苷酸序列可在啤酒酵母中将报告基因的表达降低20倍。插入的主要作用是降低前mRNA的丰度，并伴随出现3'截短的转录物，这与过早的转录终止和/或前mRNA降解相一致。顺式元件中的点突变减轻了负面影响，证明了其序列特异性。一种名为Nrd1的新型酵母蛋白和先前鉴定的推定解旋酶Sen1有助于介导顺式元件的负面作用。 Sen1是一种必需的核蛋白，与多种核功能有关。 Nrd1具有异质核糖核蛋白的特征，包括RNA识别基序，富含RE和RS二肽的区域以及富含脯氨酸和谷氨酰胺的域。 Nrd1的N末端域可能促进与RNA聚合酶II的直接相互作用。 NRD1基因的破坏是致命的，但C端截短删除了RNA识别基序并消除了顺式元件的负面影响，但仍支持细胞生长。因此，可以通过调节Nrd1的活性来调节含有顺式元件的基因的表达。哺乳动物细胞中Nrd1相关蛋白的最新鉴定表明，这种潜在的调节途径在真核生物中很普遍。

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《Molecular and Cellular Biology》 |1996年第12期|共11页
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E J Steinmetz; D A Brow;
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1. Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. [J] . E J Steinmetz, D A Brow Molecular and Cellular Biology . 1996,第12期

机译：通过与异质核核糖核蛋白样蛋白Nrd1和推定解旋酶Sen1协同作用的外源序列元件抑制基因表达。
2. Multiple factors bind the upstream activation sites of the yeast enolase genes ENO1 and ENO2: ABFI protein, like repressor activator protein RAP1, binds cis-acting sequences which modulate repression or activation of transcription. [J] . P K Brindle, J P Holland, C E Willett, Molecular and Cellular Biology . 1990,第9期

机译：多种因素结合酵母烯醇化酶基因ENO1和ENO2的上游激活位点：ABFI蛋白像阻遏物激活蛋白RAP1一样，结合可调节转录抑制或激活的顺式作用序列。
3. Involvement of a Putative Cyclic AMP Receptor Protein (CRP)-Like Binding Sequence and a CRP-Like Protein in Glucose-Mediated Catabolite Repression of thn Genes in Rhodococcus sp. Strain TFB [J] . Laura Tomás-Gallardo, Eduardo Santero, Belén Floriano Applied Microbiology . 2012,第15期

机译：假定的环AMP受体蛋白（CRP）像绑定序列和CRP像蛋白参与葡萄糖介导的Rhodococcus sp。thn基因的分解代谢抑制。应变TFB
4. Mining for putative regulatory elements in the yeast genome using gene expression data [C] . Jaak Vilo, Alvis Brazma, Inge Jonassen, International Conference on Intelligent Systems for Molecular Biology; 20000816-23; La Jolla,CA(US) . 2000

机译：使用基因表达数据挖掘酵母基因组中假定的调控元件
5. Characterization of the complete rat elastin gene: Analysis ofcis-acting elements involved in regulation of gene expression and alternative splicing and the role of repetitive sequences in gene instability. [D] . Alatawi, Abdulrahman. 1994

机译：完整的大鼠弹性蛋白基因的表征：参与基因表达调控和选择性剪接的顺式作用元件分析，以及重复序列在基因不稳定中的作用。
6. Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein Nrd1 and the putative helicase Sen1. [O] . E J Steinmetz, D A Brow 1996

机译：通过与异源核糖核蛋白样蛋白Nrd1和推定解旋酶Sen1协同作用的外源序列元件抑制基因表达。
7. Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1. [O] . Steinmetz, E J, Brow, D A 1996

机译：通过与异源核糖核蛋白样蛋白Nrd1和推定解旋酶Sen1协同作用的外源序列元件抑制基因表达。

Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1.

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