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首页> 外文期刊>Molecular and Cellular Biology >A single editing event is a prerequisite for efficient processing of potato mitochondrial phenylalanine tRNA.
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A single editing event is a prerequisite for efficient processing of potato mitochondrial phenylalanine tRNA.

机译：单个编辑事件是有效处理马铃薯线粒体苯丙氨酸tRNA的前提。

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摘要

In bean, potato, and Oenothera plants, the C encoded at position 4 (C4) in the mitochondrial tRNA Phe GAA gene is converted into a U in the mature tRNA. This nucleotide change corrects a mismatched C4-A69 base pair which appears when the gene sequence is folded into the cloverleaf structure. C-to-U conversions constitute the most common editing events occurring in plant mitochondrial mRNAs. While most of these conversions introduce changes in the amino acids specified by the mRNA and appear to be essential for the synthesis of functional proteins in plant mitochondria, the putative role of mitochondrial tRNA editing has not yet been defined. Since the edited form of the tRNA has the correct secondary and tertiary structures compared with the nonedited form, the two main processes which might be affected by a nucleotide conversion are aminoacylation and maturation. To test these possibilities, we determined the aminoacylation properties of unedited and edited potato mitochondrial tRNAPhe in vitro transcripts, as well as the processing efficiency of in vitro-synthesized potato mitochondrial tRNAPhe precursors. Reverse transcription-PCR amplification of natural precursors followed by cDNA sequencing was also used to investigate the influence of editing on processing. Our results show that C-to-U conversion at position 4 in the potato mitochondrial tRNA Phe GAA is not required for aminoacylation with phenylalanine but is likely to he essential for efficient processing of this tRNA.
机译：在豆类，马铃薯和月见草属植物中，线粒体tRNA Phe GAA基因第4位（C4）处编码的C在成熟的tRNA中转化为U。这种核苷酸改变纠正了一个错配的C4-A69碱基对，当基因序列折叠成苜蓿叶结构时出现。 C到U的转换是植物线粒体mRNA中最常见的编辑事件。虽然大多数这些转换会引入由mRNA指定的氨基酸变化，并且似乎对于植物线粒体中功能蛋白的合成至关重要，但尚未定义线粒体tRNA编辑的推定作用。由于与未编辑形式相比，tRNA的编辑形式具有正确的二级和三级结构，因此可能受到核苷酸转化影响的两个主要过程是氨基酰化和成熟。为了测试这些可能性，我们确定了未经编辑和编辑的马铃薯线粒体tRNAPhe体外转录本的氨基酰化性质，以及体外合成的马铃薯线粒体tRNAPhe前体的加工效率。天然前体的逆转录-PCR扩增，然后进行cDNA测序也用于研究编辑对加工的影响。我们的结果表明，马铃薯线粒体tRNA Phe GAA中第4位的C-U转化对于用苯丙氨酸进行氨酰化不是必需的，但对于有效处理此tRNA而言，这可能是必不可少的。

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