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首页> 外文期刊>Molecular and Cellular Biology >ch-IAP1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein.
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ch-IAP1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein.

机译：ch-IAP1是凋亡抑制蛋白家族的成员，是v-Rel癌蛋白抗凋亡活性的介质。

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The oncoprotein v-Rel, a member of the Rel/NF-kappaB family of transcription factors, induces neoplasias and inhibits apoptosis. To identify differentially regulated cellular genes and to evaluate their relevance to transformation and apoptosis in v-Rel-transformed cells, mRNA differential display has been used. One of the recovered cDNAs corresponds to a gene that was highly expressed in v-Rel-transformed fibroblasts. Analysis of the isolated full-length cDNA of a chicken inhibitor-of-apoptosis protein (ch-IAP1) revealed that it encodes a 68-kDa protein that is highly homologous to members of the IAP family, such as human c-LAP1. Like other IAPs, ch-IAP1 contains the N-terminal baculovirus IAP repeats and C-terminal RING finger motifs. Northern blot analysis identified a 3.3-kb ch-IAP1 transcript expressed at relatively high levels in the spleen, thymus, bursa, intestine, and lungs. Expression of v-Rel in fibroblasts, a B-cell line, and spleen cells up-regulated the expression of ch-IAP1. In contrast, ch-IAP1 expression levels were low in chicken cell lines transformed by several other unrelated tumor viruses. ch-IAP1 was expressed predominantly in the cytoplasm of the v-Rel-transformed cells. ch-IAP1 suppressed mammalian cell apoptosis induced by the overexpression of the interleukin-1-converting enzyme. Expression of exogenous ch-IAP1 in temperature-sensitive v-Rel transformed spleen cells inhibited apoptosis of these cells at the nonpermissive temperature. Collectively, these results suggest that ch-IAP1 is induced during the v-Rel-mediated transformation process and functions as a suppressor of apoptosis in v-Rel-transformed cells.
机译：癌蛋白v-Rel是Rel / NF-kappaB转录因子家族的成员，可诱导瘤形成并抑制细胞凋亡。为了鉴定差异调节的细胞基因并评估其与v-Rel转化细胞中转化和凋亡的相关性，已使用mRNA差异显示。回收的cDNA之一对应于在v-Rel转化的成纤维细胞中高度表达的基因。鸡凋亡抑制蛋白（ch-IAP1）的分离的全长cDNA的分析表明，它编码与IAP家族成员（例如人c-LAP1）高度同源的68 kDa蛋白。像其他IAP一样，ch-IAP1包含N端杆状病毒IAP重复序列和C端RING手指基序。 Northern印迹分析鉴定出在脾，胸腺，法氏囊，肠和肺中以相对高水平表达的3.3kb ch-IAP1转录物。 v-Rel在成纤维细胞，B细胞系和脾细胞中的表达上调了ch-IAP1的表达。相反，在由其他几种不相关的肿瘤病毒转化的鸡细胞系中，ch-IAP1表达水平较低。 ch-IAP1主要在v-Rel转化细胞的细胞质中表达。 ch-IAP1抑制了白介素1转换酶过表达诱导的哺乳动物细胞凋亡。温度敏感的v-Rel转化的脾细胞中外源性ch-IAP1的表达在非允许温度下抑制了这些细胞的凋亡。总体而言，这些结果表明，ch-IAP1在v-Rel介导的转化过程中被诱导，并在v-Rel转化的细胞中起凋亡的抑制作用。

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