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首页> 外文期刊>Molecular and Cellular Biology >Role of Histone H1 as an Architectural Determinant of Chromatin Structure and as a Specific Repressor of Transcription onXenopus Oocyte 5S rRNA Genes
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Role of Histone H1 as an Architectural Determinant of Chromatin Structure and as a Specific Repressor of Transcription onXenopus Oocyte 5S rRNA Genes

机译：组蛋白H1作为染色质结构的建筑决定因素和非洲爪蟾卵母细胞5S rRNA基因转录的特异性阻遏物的作用。

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摘要

We explore the role of histone H1 as a DNA sequence-dependent architectural determinant of chromatin structure and of transcriptional activity in chromatin. The Xenopus laevis oocyte- and somatic-type 5S rRNA genes are differentially transcribed in embryonic chromosomes in vivo depending on the incorporation of somatic histone H1 into chromatin. We establish that this effect can be reconstructed at the level of a single nucleosome. H1 selectively represses oocyte-type 5S rRNA genes by directing the stable positioning of a nucleosome such that transcription factors cannot bind to the gene. This effect does not occur on the somatic-type genes. Histone H1 binds to the 5′ end of the nucleosome core on the somatic 5S rRNA gene, leaving key regulatory elements in the promoter accessible, while histone H1 binds to the 3′ end of the nucleosome core on the oocyte 5S rRNA genes, specifically blocking access to a key promoter element (the C box). TFIIIA can bind to the somatic 5S rRNA gene assembled into a nucleosome in the presence of H1. Because H1 binds with equivalent affinities to nucleosomes containing either gene, we establish that it is the sequence-selective assembly of a specific repressive chromatin structure on the oocyte 5S rRNA genes that accounts for differential transcriptional repression. Thus, general components of chromatin can determine the assembly of specific regulatory nucleoprotein complexes.
机译：我们探索组蛋白H1作为染色质结构和染色质转录活性的DNA序列依赖的建筑决定因素的作用。爪蟾的卵母细胞和体细胞型5S rRNA基因在体内的染色体中差异转录，这取决于体细胞组蛋白H1在染色质中的掺入。我们建立了可以在单个核小体水平上重建这种效果。 H1通过指导核小体的稳定定位来选择性抑制卵母细胞型5S rRNA基因，从而使转录因子无法结合该基因。在体细胞型基因上不会发生这种影响。组蛋白H1结合到体细胞5S rRNA基因上核小体核心的5'末端，使启动子中的关键调控元件可访问，而组蛋白H1结合到卵母细胞5S rRNA基因上核小体的3'末端，特别是阻断访问密钥启动器元素（C框）。 TFIIIA可以结合在H1存在下组装成核小体的5S rRNA体细胞基因。因为H1以等同亲和力结合到包含任一基因的核小体，所以我们确定卵母细胞5S rRNA基因上特定阻抑染色质结构的序列选择性组装是造成差异转录抑制的原因。因此，染色质的一般成分可以决定特定调节性核蛋白复合物的组装。

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