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首页> 外文期刊>Molecular and Cellular Biology >A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast
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A New Member of the Sin3 Family of Corepressors Is Essential for Cell Viability and Required for Retroelement Propagation in Fission Yeast

机译：Sin3家族的新成员是细胞存活所必需的，并且是裂变酵母中逆转录因子繁殖所必需的

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Tf1 is a long terminal repeat (LTR)-containing retrotransposon that propagates within the fission yeast Schizosaccharomyces pombe. LTR-retrotransposons possess significant similarity to retroviruses and therefore serve as retrovirus models. To determine what features of the host cell are important for the proliferation of this class of retroelements, we screened for mutations in host genes that reduced the transposition activity of Tf1. We report here the isolation and characterization of pst1 +, a gene required for Tf1 transposition. The predicted amino acid sequence of Pst1p possessed high sequence homology with the Sin3 family of proteins, known for their interaction with histone deacetylases. However, unlike the SIN3 gene of Saccharomyces cerevisiae, pst1 + is essential for cell viability. Immunofluorescence microscopy indicated that Pst1p was localized in the nucleus. Consistent with the critical role previously reported for Sin3 proteins in the histone acetylation process, we found that the growth of the strain with thepst1-1 allele was supersensitive to the specific histone deacetylase inhibitor trichostatin A. However, our analysis of strains with the pst1-1 mutation was unable to detect any changes in the acetylation of specific lysines of histones H3 and H4 as measured in bulk chromatin. Interestingly, the pst1-1mutant strain produced wild-type levels of Tf1-encoded proteins and cDNA, indicating that the defect in transposition occurred after reverse transcription. The results of immunofluorescence microscopy showed that the nuclear localization of the Tf1 capsid protein was disrupted in the strain with the pst1-1mutation, indicating an important role of pst1 +in modulating the nuclear import of Tf1 virus-like particles.
机译：Tf1是一个长末端重复序列（LTR）的逆转座子，在裂殖酵母 Schizosaccharomyces pombe 中传播。 LTR-逆转座子与逆转录病毒具有显着相似性，因此可以用作逆转录病毒模型。为了确定宿主细胞的哪些特征对于此类逆转录元素的增殖很重要，我们筛选了降低Tf1转座活性的宿主基因中的突变。我们在这里报告了 pst1 + （Tf1转座所需的基因）的分离和鉴定。 Pst1p的预测氨基酸序列与Sin3蛋白质家族具有高度的序列同源性，该蛋白以与组蛋白脱乙酰基酶的相互作用而闻名。但是，与酿酒酵母（Saccharomyces cerevisiae）的 SIN3 基因不同， pst1 + 对于细胞活力至关重要。免疫荧光显微镜检查表明Pst1p位于细胞核中。与先前报道的Sin3蛋白在组蛋白乙酰化过程中的关键作用一致，我们发现带有 pst1-1 等位基因的菌株的生长对特定的组蛋白脱乙酰基酶抑制剂曲古抑菌素A非常敏感。我们对具有 pst1-1 突变的菌株的分析无法检测到以大量染色质测量的组蛋白H3和H4特定赖氨酸乙酰化的任何变化。有趣的是， pst1-1 突变株产生了野生型水平的Tf1编码的蛋白质和cDNA，表明转座缺陷发生在逆转录之后。免疫荧光显微镜检查的结果表明，带有 pst1-1 突变的菌株破坏了Tf1衣壳蛋白的核定位，表明 pst1 的重要作用+ 调节Tf1病毒样颗粒的核输入。

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