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首页> 外文期刊>Molecular and Cellular Biology >Mutations in ABO1/ELO2, a Subunit of Holo-Elongator, Increase Abscisic Acid Sensitivity and Drought Tolerance in Arabidopsis thaliana
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Mutations in ABO1/ELO2, a Subunit of Holo-Elongator, Increase Abscisic Acid Sensitivity and Drought Tolerance in Arabidopsis thaliana

机译:ABO1 / ELO2,Holo延伸剂的一个亚基的突变,增加拟南芥中的脱落酸敏感性和耐旱性。

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The phytohormone abscisic acid (ABA) plays an important role in modulating plant growth, development, and stress responses. In a genetic screen for mutants with altered drought stress responses, we identified an ABA-overly sensitive mutant, the abo1 mutant, which showed a drought-resistant phenotype. The abo1 mutation enhances ABA-induced stomatal closing and increases ABA sensitivity in inhibiting seedling growth. abo1 mutants are more resistant to oxidative stress than the wild type and show reduced levels of transcripts of several stress- or ABA-responsive genes. Interestingly, the mutation also differentially modulates the development and growth of adjacent guard cells. Map-based cloning identified ABO1 as a new allele of ELO2, which encodes a homolog of Saccharomyces cerevisiae Iki3/Elp1/Tot1 and human IκB kinase-associated protein. Iki3/Elp1/Tot1 is the largest subunit of Elongator, a multifunctional complex with roles in transcription elongation, secretion, and tRNA modification. Ecotopic expression of plant ABO1/ELO2 in a tot1/elp1Δ yeast Elongator mutant complements resistance to zymocin, a yeast killer toxin complex, indicating that ABO1/ELO2 substitutes for the toxin-relevant function of yeast Elongator subunit Tot1/Elp1. Our results uncover crucial roles for ABO1/ELO2 in modulating ABA and drought responses in Arabidopsis thaliana.
机译:植物激素脱落酸(ABA)在调节植物生长,发育和胁迫反应中起重要作用。在针对干旱胁迫响应发生变化的突变体的遗传筛选中,我们鉴定了一个 AB A- o 非常敏感的突变体,即 abo1 突变体,抗旱表型。 abo1 突变增强了ABA诱导的气孔关闭并增加了ABA在抑制幼苗生长中的敏感性。与野生型相比, abo1 突变体对氧化应激的抵抗力更高,并且显示出几种应激或ABA响应基因的转录本水平降低。有趣的是,该突变还差异地调节相邻保卫细胞的发育和生长。基于图谱的克隆鉴定了 ABO1 ELO2 的新等位基因,其编码了酿酒酵母 Iki3 / Elp1 / Tot1和人类IκB激酶的同源物相关蛋白。 Iki3 / Elp1 / Tot1是Elongator的最大亚基,Elongator是一种多功能复合物,在转录延伸,分泌和tRNA修饰中起作用。植物 ABO1 / ELO2 tot1 / elp1 Δ酵母延伸子中的异位表达可补充对酵母杀伤毒素复合物zymocin的抗性,表明 ABO1 / ELO2 < / em>替代酵母延伸子亚基Tot1 / Elp1的毒素相关功能。我们的结果揭示了ABO1 / ELO2在调节拟南芥(Arabidopsis thaliana)中的ABA和干旱响应中的关键作用。

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