Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform, Bsx1b

Hui-Yi Chu; Akihira Ohtoshi

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Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform, Bsx1b

机译：下丘脑同源盒基因Bsx1a及其同工型Bsx1b的克隆和功能分析

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The hypothalamus is a key regulatory unit of the neuroendocrine system and plays an essential role in energy balance and reproduction. Despite its important role, the molecular mechanisms underlying hypothalamic development are not fully understood. Here, we report molecular analyses of a newly identified murine homeobox gene, Bsx/Bsx1a, that is expressed in the developing and postnatal hypothalamus. We demonstrate that BSX1A is a DNA binding protein and a transcriptional activator. Transcriptional reporter assays identified the C-terminal region of BSX1A as an activation domain. We have isolated an alternative splice form of Bsx1a, designated Bsx1b, which retains the N-terminal region but lacks the homeodomain. Analyses of subcellular localization using transfected cell lines revealed that BSX1A and BSX1B localize in the nuclei and cytoplasm, respectively. Immunohistochemical analyses suggested that both BSX1A and BSX1B are expressed in the neonatal hypothalamus. Taking these data together, we propose that alternative RNA splicing is involved in hypothalamic development/function.

机译：下丘脑是神经内分泌系统的关键调节单元，在能量平衡和再生中起着至关重要的作用。尽管其重要的作用，下丘脑发育的分子机制尚未完全了解。在这里，我们报告了一个新发现的小鼠同源盒基因 Bsx / Bsx1a 的分子分析，该基因在发育中和出生后的下丘脑中表达。我们证明BSX1A是一种DNA结合蛋白和转录激活因子。转录报告基因分析鉴定出BSX1A的C端区域为激活域。我们已经分离出另一种名为 Bsx1b 的 Bsx1a 剪接形式，该剪接形式保留了N端区域，但缺少同源域。使用转染的细胞系对亚细胞定位的分析表明，BSX1A和BSX1B分别位于细胞核和细胞质中。免疫组织化学分析表明，BSX1A和BSX1B均在新生儿下丘脑中表达。综合考虑这些数据，我们建议下丘脑发育/功能涉及其他RNA剪接。 展开▼

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《Molecular and Cellular Biology》 |2007年第10期|共7页

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Hui-Yi Chu; Akihira Ohtoshi;
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中图分类细胞生物学;

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6. Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform Bsx1b [O] . Hui-Yi Chu, Akihira Ohtoshi 2007

机译：下丘脑同源盒基因Bsx1a及其同工型Bsx1b的克隆和功能分析

7. Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform, Bsx1b▿ [O] . Chu, Hui-Yi, Ohtoshi, Akihira 2007

机译：下丘脑同源盒基因Bsx1a及其同工型Bsx1b▿的克隆与功能分析

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Cloning and Functional Analysis of Hypothalamic Homeobox Gene Bsx1a and Its Isoform, Bsx1b

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