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首页> 外文期刊>Molecular and Cellular Biology >Of Bars and Rings: Hof1-Dependent Cytokinesis in Multiseptated Hyphae of Ashbya gossypii
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Of Bars and Rings: Hof1-Dependent Cytokinesis in Multiseptated Hyphae of Ashbya gossypii

机译：条和环：在Ashbya棉酚的多隔膜菌丝中Hof1依赖的细胞分裂。

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We analyzed the development of multiple septa in elongated multinucleated cells (hyphae) of the filamentous ascomycete Ashbya gossypii in which septation is apparently uncoupled from nuclear cycles. A key player for this compartmentalization is the PCH protein Hof1. Hyphae that are lacking this protein form neither actin rings nor septa but still elongate at wild-type speed. Using in vivo fluorescence microscopy, we present for the first time the coordination of cytokinesis and septation in multiseptated and multinucleated cells. Hof1, the type II myosin Myo1, the landmark protein Bud3, and the IQGAP Cyk1 form collars of cortical bars already adjacent to hyphal tips, thereby marking the sites of septation. While hyphae continue to elongate, these proteins gradually form cortical rings. This bar-to-ring transition depends on Hof1 and Cyk1 but not Myo1 and is required for actin ring assembly. The Fes/CIP4 homology (FCH) domain of Hof1 ensures efficient localization of Hof1, whereas ring integrity is conferred by the Src homology 3 (SH3) domain. Up to several hours after site selection, actin ring contraction leads to membrane invagination and subsequent cytokinesis. Simultaneously, a septum forms between the adjacent hyphal compartments, which do not separate. During evolution, A. gossypii lost the homologs of two enzymes essential for cell separation in Saccharomyces cerevisiae.
机译：我们分析了丝状子囊菌的细长多核细胞（菌丝）中多个隔膜的发育，其中分离显然与核周期无关。这种分隔的关键因素是PCH蛋白Hof1。缺少这种蛋白质的菌丝既不会形成肌动蛋白环也不会形成隔膜，但仍会以野生型速度伸长。使用体内荧光显微镜，我们首次提出了多分裂和多核细胞中胞质分裂和分离的协调。 Hof1，II型肌球蛋白Myo1，标志性蛋白Bud3和IQGAP Cyk1形成已经与菌丝尖端相邻的皮质棒状环，从而标志着分隔的部位。当菌丝继续伸长时，这些蛋白质逐渐形成皮质环。棒到环的过渡取决于Hof1和Cyk1，但不取决于Myo1，并且是肌动蛋白环组装所必需的。 Hof1的Fes / CIP4同源性（FCH）域确保了Hof1的有效定位，而环完整性由Src同源性3（SH3）域赋予。在位点选择后长达几个小时，肌动蛋白环收缩导致膜内陷和随后的胞质分裂。同时，在不分开的相邻菌丝室之间形成了隔膜。在进化过程中， A。棉酚失去了酿酒酵母中细胞分离所必需的两种酶的同源性。

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