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首页> 外文期刊>Molecular and Cellular Biology >Splicing Factor Spf30 Assists Exosome-Mediated Gene Silencing in Fission Yeast
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Splicing Factor Spf30 Assists Exosome-Mediated Gene Silencing in Fission Yeast

机译：剪接因子Spf30协助裂变酵母中外来体介导的基因沉默。

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Heterochromatin assembly in fission yeast relies on the processing of cognate noncoding RNAs by both the RNA interference and the exosome degradation pathways. Recent evidence indicates that splicing factors facilitate the cotranscriptional processing of centromeric transcripts into small interfering RNAs (siRNAs). In contrast, how the exosome contributes to heterochromatin assembly and whether it also relies upon splicing factors were unknown. We provide here evidence that fission yeast Spf30 is a splicing factor involved in the exosome pathway of heterochromatin silencing. Spf30 and Dis3, the main exosome RNase, colocalize at centromeric heterochromatin and euchromatic genes. At the centromeres, Dis3 helps recruiting Spf30, whose deficiency phenocopies the dis3-54 mutant: heterochromatin is impaired, as evidenced by reduced silencing and the accumulation of polyadenylated centromeric transcripts, but the production of siRNAs appears to be unaffected. Consistent with a direct role, Spf30 binds centromeric transcripts and locates at the centromeres in an RNA-dependent manner. We propose that Spf30, bound to nascent centromeric transcripts, perhaps with other splicing factors, assists their processing by the exosome. Splicing factor intercession may thus be a common feature of gene silencing pathways.
机译：裂变酵母中的异染色质装配依赖于通过RNA干扰和外泌体降解途径对同源非编码RNA的加工。最近的证据表明，剪​​接因子促进着丝粒转录本向小干扰RNA（siRNA）的共转录加工。相反，外泌体如何促进异染色质组装以及是否也依赖剪接因子尚不清楚。我们在此处提供的证据表明，裂变酵母Spf30是异染色质沉默的外泌体途径中涉及的剪接因子。 Spf30和Dis3是主要的外泌体RNase，共定位于着丝粒异染色质和常染色体基因。在着丝粒处，Dis3帮助募集Spf30，其缺陷表型复制 dis3-54 突变体：异染色质受损，这通过减少沉默和聚腺苷酸着丝粒转录物的积累来证明，但siRNA的产生似乎是不受影响。与直接作用一致，Spf30结合着丝粒转录物并以RNA依赖性方式定位着丝粒。我们建议将Spf30与新生的着丝粒转录本结合，也许还结合其他剪接因子，以协助外泌体对其进行加工。因此，剪接因子代祷可能是基因沉默途径的共同特征。

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