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首页> 外文期刊>Molecular and Cellular Biology >Control of Chromatin Structure by Spt6: Different Consequences in Coding and Regulatory Regions
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Control of Chromatin Structure by Spt6: Different Consequences in Coding and Regulatory Regions

机译：通过Spt6控制染色质结构：编码和监管区域的不同后果。

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Spt6 is a highly conserved factor required for normal transcription and chromatin structure. To gain new insights into the roles of Spt6, we measured nucleosome occupancy along Saccharomyces cerevisiae chromosome III in an spt6 mutant. We found that the level of nucleosomes is greatly reduced across some, but not all, coding regions in an spt6 mutant, with nucleosome loss preferentially occurring over highly transcribed genes. This result provides strong support for recent studies that have suggested that transcription at low levels does not displace nucleosomes, while transcription at high levels does, and adds the idea that Spt6 is required for restoration of nucleosomes at the highly transcribed genes. Unexpectedly, our studies have also suggested that the spt6 effects on nucleosome levels across coding regions do not cause the spt6 effects on mRNA levels, suggesting that the role of Spt6 across coding regions is separate from its role in transcriptional regulation. In the case of the CHA1 gene, regulation by Spt6 likely occurs by controlling the position of the +1 nucleosome. These results, along with previous studies, suggest that Spt6 regulates transcription by controlling chromatin structure over regulatory regions, and its effects on nucleosome levels over coding regions likely serve an independent function.
机译：Spt6是正常转录和染色质结构所需的高度保守因子。为了获得对Spt6的作用的新见解，我们测量了 spt6 突变体中啤酒酵母（Saccharomyces cerevisiae）染色体III沿核小体的占用。我们发现， spt6 突变体中部分而非全部编码区的核小体水平大大降低，而核小体的丢失优先于高度转录的基因。该结果为最近的研究提供了有力的支持，这些研究表明低水平的转录不会取代核小体，而高水平的转录却能替代核小体，并增加了Spt6是高转录基因还原核小体所必需的想法。出乎意料的是，我们的研究还表明， spt6 对整个编码区的核小体水平的影响不会导致 spt6 对mRNA水平的影响，这表明Spt6在整个编码区的作用与它在转录调控中的作用是分开的。在 CHA1 基因的情况下，Spt6的调控可能是通过控制+1核小体的位置来进行的。这些结果以及以前的研究表明，Spt6通过控制整个调控区域的染色质结构来调控转录，其对编码区域核小体水平的影响可能起着独立的作用。

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