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Glycolysis Controls Plasma Membrane Glucose Sensors To Promote Glucose Signaling in Yeasts

机译:糖酵解控制血浆膜葡萄糖传感器促进酵母中的葡萄糖信号传递

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Sensing of extracellular glucose is necessary for cells to adapt to glucose variation in their environment. In the respiratory yeast Kluyveromyces lactis, extracellular glucose controls the expression of major glucose permease gene RAG1 through a cascade similar to the Saccharomyces cerevisiae Snf3/Rgt2/Rgt1 glucose signaling pathway. This regulation depends also on intracellular glucose metabolism since we previously showed that glucose induction of the RAG1 gene is abolished in glycolytic mutants. Here we show that glycolysis regulates RAG1 expression through the K. lactis Rgt1 (KlRgt1) glucose signaling pathway by targeting the localization and probably the stability of Rag4, the single Snf3/Rgt2-type glucose sensor of K. lactis. Additionally, the control exerted by glycolysis on glucose signaling seems to be conserved in S. cerevisiae. This retrocontrol might prevent yeasts from unnecessary glucose transport and intracellular glucose accumulation.
机译:细胞外葡萄糖的感觉对于细胞适应环境中的葡萄糖变化是必要的。在呼吸酵母乳酸克鲁维酵母中,细胞外葡萄糖通过类似于酿酒酵母Snf3 / Rgt2 / Rgt1葡萄糖信号传导途径的级联控制主要葡萄糖通透酶基因 RAG1 的表达。该调节还取决于细胞内的葡萄糖代谢,因为我们之前已经证明 RAG1 基因的葡萄糖诱导在糖酵解突变体中被取消。在这里,我们显示糖酵解通过定位乳酸克鲁格酵母Rgt1( Kl Rgt1)葡萄糖信号传导通路来调节 RAG1 表达,并可能定位到Rag4(单个Snf3 /乳酸克鲁维酵母的Rgt2型葡萄糖传感器。另外,糖酵解对葡萄糖信号传导施加的控制似乎在酿酒酵母中是保守的。这种逆向控制可以防止酵母不必要的葡萄糖转运和细胞内葡萄糖积累。

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