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首页> 外文期刊>Molecular and Cellular Biology >A Pcl-Like Cyclin of Aspergillus nidulans Is Transcriptionally Activated by Developmental Regulators and Is Involved in Sporulation
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A Pcl-Like Cyclin of Aspergillus nidulans Is Transcriptionally Activated by Developmental Regulators and Is Involved in Sporulation

机译：构巢曲霉的Pcl样细胞周期蛋白被发育调节剂转录激活并参与孢子形成。

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The filamentous fungus Aspergillus nidulansreproduces asexually through the formation of spores on a multicellular aerial structure, called a conidiophore. A key regulator of asexual development is the TFIIIA-type zinc finger containing transcriptional activator Bristle (BRLA). Besides BRLA, the transcription factor ABAA, which is located downstream of BRLA in the developmental regulation cascade, is necessary to direct later gene expression during sporulation. We isolated a new developmental mutant and identified a leaky brlA mutation and the mutatedSaccharomyces cerevisiae cyclin homologuepclA, both contributing to the developmental phenotype of the mutant. pclA was found to be 10-fold transcriptionally upregulated during conidiation, and apclA deletion strain was reduced three- to fivefold in production of conidia. Expression of pclA was strongly induced by ectopic expression of brlA orabaA under conidiation-suppressing conditions, indicating a direct role for brlA andabaA in pclA regulation. PCLA is homologous to yeast Pcl cyclins, which interact with the Pho85 cyclin-dependent kinase. Although interaction with a PSTAIRE kinase was shown in vivo, PCLA function during sporulation was independent of theA. nidulans Pho85 homologue PHOA. Besides the developmental regulation, pclA expression was cell cycle dependent with peak transcript levels in S phase. Our findings suggest a role for PCLA in mediating cell cycle events during late stages of sporulation.
机译：丝状真菌构巢曲霉通过多孢子空中结构上的孢子形成而无性繁殖。无性发育的关键调节因子是TFIIIA型锌指，其中含有转录激活剂Bristle（BRLA）。除了BRLA 外，转录因子ABAA位于发育调控级联反应中BRLA的下游，对于指导孢子形成过程中以后的基因表达也是必需的。我们分离出一个新的发育突变体，并鉴定出一个泄漏的 brlA 突变和突变的酿酒酵母细胞周期蛋白同系物 pclA ，它们都有助于该基因的发育表型。突变体。发现 pclA 在分生孢子过程中转录上调了10倍，而 pclA 缺失菌株在分生孢子的产生中减少了三到五倍。 brlA 或 abaA 在异位抑制条件下的异位表达强烈诱导 pclA 的表达，表明 brlA < / em>和 abaA 在 pclA 法规中。 PCLA与酵母Pcl细胞周期蛋白同源，后者与Pho85细胞周期蛋白依赖性激酶相互作用。尽管在体内显示了与PSTAIRE激酶的相互作用，但孢子形成过程中的PCLA功能独立于emA。 nidulans Pho85同源物PHOA。除发育调控外， pclA 的表达还依赖于细胞周期，其S期的转录本水平最高。我们的发现表明，PCLA在孢子形成后期可在介导细胞周期事件中发挥作用。

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