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首页> 外文期刊>Molecular and Cellular Biology >Mechanisms Controlling Subcellular Localization of the G1 Cyclins Cln2p and Cln3p in Budding Yeast
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Mechanisms Controlling Subcellular Localization of the G1 Cyclins Cln2p and Cln3p in Budding Yeast

机译：控制芽孢酵母中G1细胞周期蛋白Cln2p和Cln3p亚细胞定位的机制。

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Different G1 cyclins confer functional specificity to the cyclin-dependent kinase (Cdk) Cdc28p in budding yeast. The Cln3p G1 cyclin is localized primarily to the nucleus, while Cln2p is localized primarily to the cytoplasm. Both binding to Cdc28p and Cdc28p-dependent phosphorylation in the C-terminal region of Cln2p are independently required for efficient nuclear depletion of Cln2p, suggesting that this process may be physiologically regulated. The accumulation of hypophosphorylated Cln2 in the nucleus is an energy-dependent process, but may not involve the RAN GTPase. Phosphorylation of Cln2p is inefficient in small newborn cells obtained by elutriation, and this lowered phosphorylation correlates with reduced Cln2p nuclear depletion in newborn cells. Thus, Cln2p may have a brief period of nuclear residence early in the cell cycle. In contrast, the nuclear localization pattern of Cln3p is not influenced by Cdk activity. Cln3p localization requires a bipartite nuclear localization signal (NLS) located at the C terminus of the protein. This sequence is required for nuclear localization of Cln3p and is sufficient to confer nuclear localization to green fluorescent protein in a RAN-dependent manner. Mislocalized Cln3p, lacking the NLS, is much less active in genetic assays specific for Cln3p, but more active in assays normally specific for Cln2p, consistent with the idea that Cln3p localization explains a significant part of Clnp functional specificity.
机译：不同的G 1 细胞周期蛋白赋予芽生酵母细胞周期蛋白依赖性激酶（Cdk）Cdc28p功能特异性。 Cln3p G 1 细胞周期蛋白主要定位于细胞核，而Cln2p主要定位于细胞质。 Cln2p的C末端区域的Cdc28p和Cdc28p依赖性磷酸化的Cdc28p依赖的磷酸化的结合对于Cln2p的有效核耗尽都是独立需要的，这表明该过程可能是生理调节的。次磷酸化Cln2在细胞核中的积累是能量依赖的过程，但可能不涉及RAN GTPase。在通过淘洗获得的小型新生细胞中，Cln2p的磷酸化效率低下，而这种降低的磷酸化与新生细胞中减少的Cln2p核耗竭相关。因此，Cln2p在细胞周期的早期可能有短暂的核滞留期。相反，Cln3p的核定位模式不受Cdk活性的影响。 Cln3p定位需要位于蛋白质C末端的二分核定位信号（NLS）。该序列对于Cln3p的核定位是必需的，并且足以以RAN依赖的方式赋予绿色荧光蛋白核定位。缺少NLS的错误定位的Cln3p在对Cln3p特异的基因检测中活性低得多，但在通常对Cln2p特异的检测中活性较高，这与Cln3p本地化解释了Clnp功能特异性的重要意义相一致。

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