The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene

Laura N. Rusché; Catherine E. Huang; Kenneth J. Piller; Michael Hemann; Elizabeth Wirtz; Barbara Sollner-Webb

首页> 外文期刊>Molecular and Cellular Biology >The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene

【24h】

The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene

机译：布鲁氏锥虫RNA编辑复合体的两个RNA Ligases：克隆必需的带IV基因并鉴定带V基因

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Kinetoplastid RNA editing is a posttranscriptional insertion and deletion of U residues in mitochondrial transcripts that involves RNA ligase. A complex of seven different polypeptides purified fromTrypanosoma brucei mitochondria that catalyzes accurate RNA editing contains RNA ligases of ～57 kDa (band IV) and ～50 kDa (band V). From a partial amino acid sequence, cDNA and genomic clones of band IV were isolated, making it the first cloned component of the minimal RNA editing complex. It is indeed an RNA ligase, for when expressed inEscherichia coli, the protein autoadenylylates and catalyzes RNA joining. Overexpression studies revealed that T. brucei can regulate of total band IV protein at the level of translation or protein stability, even upon massively increased mRNA levels. The protein's mitochondrial targeting was confirmed by its location, size when expressed in T. brucei and E. coli, and N-terminal sequence. Importantly, genetic knockout studies demonstrated that the gene for band IV is essential in procyclic trypanosomes. The band IV and band V RNA ligases of the RNA editing complex therefore serve different functions. We also identified the gene for band V RNA ligase, a protein much more homologous to band IV than to other known ligases.

机译：运动质体RNA编辑是涉及RNA连接酶的线粒体转录物中U残基的转录后插入和缺失。从布鲁氏锥虫线粒体纯化的七种不同多肽的复合物可催化准确的RNA编辑，其中包含〜57 kDa（IV带）和〜50 kDa（V带）的RNA连接酶。从部分氨基酸序列中，分离出IV带的cDNA和基因组克隆，使其成为最小RNA编辑复合体的第一个克隆成分。它确实是一种RNA连接酶，因为当它在大肠杆菌中表达时，蛋白质自身腺苷酸化并催化RNA连接。过度表达研究表明， T。 brucei 即使在mRNA水平大量增加的情况下，也可以在翻译或蛋白质稳定性水平上调节IV总蛋白。蛋白质的线粒体靶向性通过在 T中表达时的位置和大小来确定。 brucei 和 E。大肠杆菌和N端序列。重要的是，基因敲除研究表明，IV带基因在前环锥虫中至关重要。因此，RNA编辑复合体的IV带和V带RNA连接酶具有不同的功能。我们还鉴定了V带RNA连接酶的基因，该蛋白与IV带比与其他已知连接酶同源得多的蛋白质。 展开▼

著录项

来源
《Molecular and Cellular Biology》 |2001年第4期|共11页

作者
Laura N. Rusché; Catherine E. Huang; Kenneth J. Piller; Michael Hemann; Elizabeth Wirtz; Barbara Sollner-Webb;
展开▼

作者单位

展开▼

收录信息

原文格式 PDF

正文语种

中图分类细胞生物学;

关键词

相似文献

外文文献

中文文献

专利

1. Trypanosoma brucei RNA Editing Complex Band II Is Structurally Critical and Maintains Band V Ligase, Which Is Nonessential [J] . Sean F. OHearn, Catherine E. Huang, Mike Hemann, Molecular and Cellular Biology . 2003,第21期

机译：布氏锥虫RNA编辑复合带II在结构上至关重要，并维持V带连接酶，这是不必要的

2. Assembly and Function of the RNA Editing Complex in Trypanosoma brucei Requires Band III Protein [J] . Catherine E. Huang, Sean F. OHearn, Barbara Sollner-Webb Molecular and Cellular Biology . 2002,第9期

机译：布鲁氏锥虫RNA编辑复合体的组装和功能需要Band III蛋白。

3. Assembly and Function of the RNA Editing Complex in Trypanosoma brucei Requires Band III Protein [J] . Catherine E. Huang, Sean F. OHearn, Barbara Sollner-Webb Molecular and Cellular Biology . 2002,第9期

机译：布鲁氏锥虫RNA编辑复合体的组装和功能需要Band III蛋白。

4. In-Silico Studies of Essential Metabolic Reactions of Trypanosoma brucei: Identifying Potential Drug Targets [C] . Shalom Nwodo Chinedu, Segun A. Fatumo, Ezekiel F. Adebiyi International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics . 2010

机译：硅瘤瘤菌瘤菌瘤的基本代谢反应的硅研究：鉴定潜在的药物靶标

5. The roles of three critical proteins in the structure and function of the Trypanosoma brucei RNA editing complex. [D] . Law, Julie Ann. 2007

机译：三个关键蛋白在布鲁氏锥虫RNA编辑复合体的结构和功能中的作用。

6. The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene [O] . Laura N. Rusché, Catherine E. Huang, Kenneth J. Piller, 2001

机译：布鲁氏锥虫RNA编辑复合体的两个RNA Ligases：克隆必需的带IV基因并鉴定带V基因

7. The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene [O] . Rusché, Laura N., Huang, Catherine E., Piller, Kenneth J., 2001

机译：布鲁氏锥虫RNA编辑复合体的两个RNA Ligases：克隆必需的带IV基因并鉴定带V基因

1. 布氏锥虫苯丙氨酰-tRNA合成酶在大肠杆菌中的克隆、表达、纯化及活性测定 [J] . 姚璎 ,杲光伟 ,李大伟 . 生物工程学报 . 2010,第001期

2. 布氏锥虫亮氨酰-tRNA合成酶的克隆表达纯化及活性测定 [J] . 姚璎 ,杲光伟 ,李大伟 . 中国生化药物杂志 . 2010,第001期

3. 苹果两个新S-RNase基因克隆与46个品种S基因型的PCR分析 [J] . 龙慎山 ,李茂福 ,韩振海 . 农业生物技术学报 . 2010,第002期

4. RNA测序联合CRISPR/Cas9基因编辑技术鉴定调控维甲酸诱导分化功能的miRNA [J] . 王凌燕 ,林仁章 ,江佩芳 . 中国实验血液学杂志 . 2021,第002期

5. 小菜蛾U6 snRNA基因的克隆及作为miRNA定量表达分析内参基因的评价 [J] . 封冰 ,梁沛 ,高希武 . 昆虫学报 . 2014,第003期

6. 伊氏锥虫TERT基因克隆及鉴定 [C] . 赵娜 ,宫鹏涛 ,李建华 . 中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会 . 2013

7. 蜜蜂、家蚕ADAR基因克隆和在毕赤酵母中表达研究及昆虫钠离子通道基因克隆、选择性剪切及RNA编辑分析 [A] . 吴晓杰 . 2008

1. 一种以RNA介导的特异性敲除双基因获得基因编辑绵羊的方法及其专用sgRNA [P] . 中国专利： CN105132427B . 2019.01.08

2. 一种以RNA介导的特异性敲除FGF5基因获得基因编辑绵羊的方法及其专用sgRNA [P] . 中国专利： CN105132426B . 2018.01.09

3. Site-specific RNA mutagenesis method, target editing guide RNA used therefor, and target RNA-target editing guide RNA complex [P] . 外国专利： JP6624743B2 . 2019-12-25

机译：位点特异性RNA诱变方法，用于其的靶标编辑指南RNA以及靶标RNA-靶标编辑指南RNA复合物

4. Site-specific RNA mutagenesis method, target editing guide RNA used therefor, and target RNA-target editing guide RNA complex [P] . 外国专利： JPWO2017010556A1 . 2018-06-21

机译：位点特异性RNA诱变方法，用于其的靶标编辑指南RNA以及靶标RNA-靶标编辑指南RNA复合物

5. Genetically engineered hepatitis c virus (hcv) nucleic acid clone, plasmid clone that houses a life-size hcv cdna, dna or rna transcribed from the full-length hcv cdna, transfected and in vitro infected host cell line with hcv, non-human animal infected with hcv, hcv viral particle, in vitro test system, hcv cell free, hcv vaccine and hcv test set and processes for identifying a cell line that is permissive and an animal that is permissive for hcv infection, to select hcv with adaptive mutations that allow higher levels of hcv replication in a permissive cell line, to infect an animal with hcv, to spread hcv in vitro, to transduce an animal susceptible to hcv infection with a heterologous gene, to produce hcv viral particles, hcv particle proteins and hcv antibodies, to separate agents capable of to modulate hcv replication, to prepare a hcv nucleic acid and to detect hcv antibodies in a biological sample from a patient. [P] . 外国专利： BR9808147A . 2000-03-28

机译：基因工程丙型肝炎病毒（hcv）核酸克隆，质粒克隆，其中包含从全长hcv cdna转录而来的真人大小的hcv cdna，dna或rna，并通过非人类动物的hcv转染和体外感染宿主细胞系被hcv感染，hcv病毒颗粒，体外测试系统，无hcv细胞，hcv疫苗和hcv测试仪的感染以及鉴定允许的细胞系和允许感染hvv的动物的过程，以选择具有以下适应性突变的hcv：允许在允许的细胞系中进行更高水平的hvv复制，以hvv感染动物，在体外传播hvv，用异源基因转导易感染hvv的动物，产生hvv病毒颗粒，hvv颗粒蛋白和hvv抗体分离能够调节hcv复制的试剂，制备hcv核酸并检测患者生物样品中的hcv抗体。

相关主题

The Two RNA Ligases of the Trypanosoma brucei RNA Editing Complex: Cloning the Essential Band IV Gene and Identifying the Band V Gene

摘要

著录项

相似文献

相关主题

期刊订阅