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首页> 外文期刊>Molecular and Cellular Biology >Swapping Functional Specificity of a MADS Box Protein: Residues Required for Arg80 Regulation of Arginine Metabolism
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Swapping Functional Specificity of a MADS Box Protein: Residues Required for Arg80 Regulation of Arginine Metabolism

机译：交换MADS盒蛋白的功能特异性：精氨酸代谢的Arg80调节所需的残基。

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摘要

Arg80 and Mcm1, two members of the MADS box family of DNA-binding proteins, regulate the metabolism of arginine in association with Arg81, the arginine sensor. In spite of the high degree of sequence conservation between the MADS box domains of the Arg80 and Mcm1 proteins (56 of 81 amino acids), these domains are not interchangeable. To determine which amino acids define the specificity of Arg80, we swapped the amino acids in each secondary-structure element of the Arg80 MADS box domain with the corresponding amino acids of Mcm1 and assayed the ability of these chimeras to regulate arginine-metabolic genes in place of the wild-type Arg80. Also performed was the converse experiment in which each variant residue in the Mcm1 MADS box domain was swapped with the corresponding residue of Arg80 in the context of an Arg80-Mcm1 fusion protein. We show that multiple regions of Arg80 are important for its function. Interestingly, the residues which have important roles in determining the specificity of Arg80 are not those which could contact the DNA but are residues that are likely to be involved in protein interactions. Many of these residues are clustered on one side of the protein, which could serve as an interface for interaction with Arg81 or Mcm1. This interface is distinct from the region used by the Mcm1 and human serum response factor MADS box proteins to interact with their cofactors. It is possible that this alternative interface is used by other MADS box proteins to interact with their cofactors.
机译：DNA结合蛋白MADS盒家族的两个成员Arg80和Mcm1与精氨酸传感器Arg81一起调节精氨酸的代谢。尽管Arg80和Mcm1蛋白的MADS框结构域（81个氨基酸中的56个）之间的序列保守性很高，但这些结构域不可互换。为了确定哪些氨基酸定义了Arg80的特异性，我们将Arg80 MADS盒结构域的每个二级结构元件中的氨基酸与相应的Mcm1氨基酸进行了交换，并分析了这些嵌合体调节原位精氨酸代谢基因的能力。是野生型Arg80。还进行了相反的实验，其中在Arg80-Mcm1融合蛋白的情况下，Mcm1 MADS盒结构域中的每个变异残基都与Arg80的相应残基交换。我们表明，Arg80的多个区域对其功能很重要。有趣的是，在确定Arg80的特异性中具有重要作用的残基不是可能与DNA接触的残基，而是可能参与蛋白质相互作用的残基。这些残基中的许多残基都聚集在蛋白质的一侧，可以用作与Arg81或Mcm1相互作用的界面。该界面不同于Mcm1和人血清反应因子MADS框蛋白与其辅因子相互作用的区域。其他MADS盒蛋白可能会使用此替代界面来与其辅因子相互作用。

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