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首页> 外文期刊>Molecular and Cellular Biology >YB-1 Autoregulates Translation of Its Own mRNA at or prior to the Step of 40S Ribosomal Subunit Joining
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YB-1 Autoregulates Translation of Its Own mRNA at or prior to the Step of 40S Ribosomal Subunit Joining

机译：YB-1在40S核糖体亚基加入步骤或之前自动调节其自身mRNA的翻译

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YB-1 is a member of the numerous families of proteins with an evolutionary ancient cold-shock domain. It is involved in many DNA- and RNA-dependent events and regulates gene expression at different levels. Previously, we found a regulatory element within the 3′ untranslated region (UTR) of YB-1 mRNA that specifically interacted with YB-1 and poly(A)-binding protein (PABP); we also showed that PABP positively affected YB-1 mRNA translation in a poly(A) tail-independent manner (O. V. Skabkina, M. A. Skabkin, N. V. Popova, D. N. Lyabin, L. O. Penalva, and L. P. Ovchinnikov, J. Biol. Chem. >278:18191-18198, 2003). Here, YB-1 is shown to strongly and specifically inhibit its own synthesis at the stage of initiation, with accumulation of its mRNA in the form of free mRNPs. YB-1 and PABP binding sites have been mapped on the YB-1 mRNA regulatory element. These were UCCAG/ACAA for YB-1 and a ～50-nucleotide A-rich sequence for PABP that overlapped each other. PABP competes with YB-1 for binding to the YB-1 mRNA regulatory element and restores translational activity of YB-1 mRNA that has been inhibited by YB-1. Thus, YB-1 negatively regulates its own synthesis, presumably by specific interaction with the 3′UTR regulatory element, whereas PABP restores translational activity of YB-1 mRNA by displacing YB-1 from this element.
机译：YB-1是具有进化古代冷休克结构域的众多蛋白质家族的成员。它参与许多依赖DNA和RNA的事件，并在不同水平上调节基因表达。以前，我们在YB-1 mRNA的3'非翻译区（UTR）中发现了一个与YB-1和poly（A）结合蛋白（PABP）特异性相互作用的调控元件;我们还表明，PABP以不依赖尾部的方式（A Skabkina，MA Skabkin，NV Popova，DN Lyabin，LO Penalva和LP Ovchinnikov，J. Biol。Chem。 > 278： 18191-18198，2003）。在此，显示YB-1在起始阶段强烈且特异性地抑制其自身的合成，其mRNA以游离mRNP的形式积累。 YB-1和PABP结合位点已被映射在YB-1 mRNA调控元件上。它们分别是YB-1的UCCAG / ACAA和PABP的〜50核苷酸富集序列，它们相互重叠。 PABP与YB-1竞争与YB-1 mRNA调控元件的结合，并恢复已被YB-1抑制的YB-1 mRNA的翻译活性。因此，YB-1可能通过与3'UTR调控元件的特异性相互作用来负调控自身的合成，而PABP通过从该元件上置换YB-1来恢复YB-1 mRNA的翻译活性。

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