The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity

Rakesh K. Mishra; Jozsef Mihaly; Stéphane Barges; Annick Spierer; Fran?ois Karch; Kirsten Hagstrom; Susan E. Schweinsberg; Paul Schedl

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The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity

机译：iab-7多梳响应元件映射到染色质的无核小体区域，并且需要GAGA和Pleiohomeotic才能沉默。

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In the work reported here we have undertaken a functional dissection of a Polycomb response element (PRE) from the iab-7 cis-regulatory domain of the Drosophila melanogasterbithorax complex (BX-C). Previous studies mapped the iab-7PRE to an 860-bp fragment located just distal to the Fab-7boundary. Located within this fragment is an ～230-bp chromatin-specific nuclease-hypersensitive region called HS3. We have shown that HS3 is capable of functioning as a Polycomb-dependent silencer in vivo, inducing pairing-dependent silencing of amini-white reporter. The HS3 sequence contains consensus binding sites for the GAGA factor, a protein implicated in the formation of nucleosome-free regions of chromatin, and Pleiohomeotic (Pho), a Polycomb group protein that is related to the mammalian transcription factor YY1. We show that GAGA and Pho interact with these sequences in vitro and that the consensus binding sites for the two proteins are critical for the silencing activity of theiab-7 PRE in vivo.

机译：在这里报道的工作中，我们已经从果蝇果蝇复合体（BX）的 iab-7顺式调节域对Polycomb反应元件（PRE）进行了功能解剖。 -C）。以前的研究将 iab-7 PRE映射到一个位于 Fab-7 边界远端的860 bp片段。该片段内有一个约230 bp的染色质特异性核酸酶超敏感区，称为HS3。我们已经表明，HS3能够在体内充当依赖于聚梳的沉默子，诱导 mini-white 报告基因的配对依赖沉默。 HS3序列包含GAGA因子（一种涉及染色质无核小体区域形成的蛋白）和Pleiohomeotic（Pho）（一种与哺乳动物转录因子YY1相关的多梳基团蛋白）的共有结合位点。我们显示GAGA和Pho在体外与这些序列相互作用，并且这两种蛋白的共有结合位点对于体内 iab-7 PRE的沉默活性至关重要。 展开▼

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《Molecular and Cellular Biology》 |2001年第4期|共8页

作者
Rakesh K. Mishra; Jozsef Mihaly; Stéphane Barges; Annick Spierer; Fran?ois Karch; Kirsten Hagstrom; Susan E. Schweinsberg; Paul Schedl;
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中图分类细胞生物学;

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1. GAGA facilitates binding of Pleiohomeotic to a chromatinized Polycomb response element [J] . Tokameh Mahmoudi, Lobke M. P. Zuijderduijn, Adone Mohd-Sarip, Nucleic Acids Research . 2003,第14期

机译：GAGA促进Pleiohomeotic与染色质Polycomb反应元件的结合

2. The bithorax complex iab-7 Polycomb response element has a novel role in the functioning of the Fab-7 chromatin boundary [J] . Olga Kyrchanova, Amina Kurbidaeva, Marat Sabirov, PLoS Genetics . 2018,第8期

机译：Bithorax复合物 iab-7 聚梳响应元件在 Fab-7 染色质边界的功能中具有新作用

3. The bithorax complex iab-7 Polycomb response element has a novel role in the functioning of the Fab-7 chromatin boundary [J] . Olga Kyrchanova, Amina Kurbidaeva, Marat Sabirov, PLoS Genetics . 2018,第8期

机译：Bithorax复合物 iab-7 聚梳响应元件在 Fab-7 染色质边界的功能中具有新作用

4. Discovering distal regulatory elements by integrating multiple types of chromatin state maps [C] . Teng Li, Tan Kai 2012 IEEE International Conference on Bioinformatics and Biomedicine. . 2012

机译：通过整合多种类型的染色质状态图来发现远端调控元件

5. Mapping replication and silencing suppression elements in the RCNMV genome. [D] . Powers, Jason Gannon. 2009

机译：映射RCNMV基因组中的复制和沉默抑制元件。

6. The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity [O] . Rakesh K. Mishra, Jozsef Mihaly, Stéphane Barges, 2001

机译：iab-7聚梳响应元件映射到染色质的无核小体区域并且需要GAGA和Pleiohomeotic才能沉默。

7. The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity [O] . Mishra, Rakesh K., Mihaly, Jozsef, Barges, Stéphane, 2001

机译：iab-7聚梳响应元件映射到染色质的无核小体区域，并且需要GAGA和Pleiohomeotic才能沉默。

1. Recombinant proteins having thrombomodulin activity, DNA constructs which encode them, vectors, cells and process for preparing them, pharmaceutical preparations which comprise the proteins, and the use of the proteins for coating /HO The invention comprises a group of compounds which possess thrombomodulin activity defined by a high degree of affinity binding to thrombin and the ability to provide a complex between such a compound and thrombin having the ability to activate protein C, and which comprise, from the C-terminal moiety of the molecule, two or more of the following structural elements: a) a short cytoplasmic domain of approx. 56 amino acids, b) a transmembrane region of approx. 24 amino acids, c) a region which is rich in serine, threonine and proline residues, d) a domain which comprises at least two EGF domains, and e) a N-terminal domain, where one or more of the elements a), b) and/or c) can be omitted or replaced with affinity-imparting units or units which inc [P] . 外国专利： DK162169B . 1991-09-23

机译：具有血栓调节蛋白活性的重组蛋白，编码它们的DNA构建体，载体，细胞及其制备方法，包含该蛋白质的药物制剂以及该蛋白用于包被的用途。本发明包括一组具有血栓调节蛋白的化合物活性由与凝血酶的高度亲和力结合以及在此类化合物与凝血酶之间提供复合物的能力定义，该凝血酶具有激活蛋白C的能力，并且从分子的C末端部分包含两个或多个以下结构元件：a）约一个短的胞质结构域。 56个氨基酸，b）跨膜区24个氨基酸，c）富含丝氨酸，苏氨酸和脯氨酸残基的区域，d）包含至少两个EGF结构域的结构域，e）N末端结构域，其中一个或多个元素a）， b）和/或c）可以省略或替换为赋予亲和力的单位或包含

2. Exhaust gas line for an internal combustion engine comprises catalyst support with regions having different physical and/or chemical properties in relation to response behavior, permeability, catalytic activity and/or thermal inertia [P] . 外国专利： AT501338B1 . 2008-02-15

机译：用于内燃发动机的排气管线包括催化剂载体，该催化剂载体具有相对于响应行为，渗透性，催化活性和/或热惯性而言具有不同物理和/或化学性质的区域。

3. Exhaust gas line for an internal combustion engine comprises catalyst support with regions having different physical and/or chemical properties in relation to response behavior, permeability, catalytic activity and/or thermal inertia [P] . 外国专利： AT501338A2 . 2006-08-15

机译：用于内燃发动机的排气管线包括催化剂载体，该催化剂载体具有相对于响应行为，渗透性，催化活性和/或热惯性而言具有不同物理和/或化学性质的区域。

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The iab-7 Polycomb Response Element Maps to a Nucleosome-Free Region of Chromatin and Requires Both GAGA and Pleiohomeotic for Silencing Activity

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