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首页> 外文期刊>Molecular and Cellular Biology >Nup358/RanBP2 Attaches to the Nuclear Pore Complex via Association with Nup88 and Nup214/CAN and Plays a Supporting Role in CRM1-Mediated Nuclear Protein Export
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Nup358/RanBP2 Attaches to the Nuclear Pore Complex via Association with Nup88 and Nup214/CAN and Plays a Supporting Role in CRM1-Mediated Nuclear Protein Export

机译：Nup358 / RanBP2通过与Nup88和Nup214 / CAN缔合而附着于核孔复合体，并在CRM1介导的核蛋白出口中发挥支持作用。

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Nuclear pore complexes (NPCs) traverse the nuclear envelope (NE), providing a channel through which nucleocytoplasmic transport occurs. Nup358/RanBP2, Nup214/CAN, and Nup88 are components of the cytoplasmic face of the NPC. Here we show that Nup88 localizes midway between Nup358 and Nup214 and physically interacts with them. RNA interference of either Nup88 or Nup214 in human cells caused a strong reduction of Nup358 at the NE. Nup88 and Nup214 showed an interdependence at the NPC and were not affected by the absence of Nup358. These data indicate that Nup88 and Nup214 mediate the attachment of Nup358 to the NPC. We show that localization of the export receptor CRM1 at the cytoplasmic face of the NE is Nup358 dependent and represents its empty state. Also, removal of Nup358 causes a distinct reduction in nuclear export signal-dependent nuclear export. We propose that Nup358 provides both a platform for rapid disassembly of CRM1 export complexes and a binding site for empty CRM1 recycling into the nucleus.
机译：核孔复合物（NPC）穿过核被膜（NE），提供发生核质转运的通道。 Nup358 / RanBP2，Nup214 / CAN和Nup88是NPC细胞质表面的组成部分。在这里，我们显示Nup88定位在Nup358和Nup214之间的中间位置，并与其物理交互。 Nup88或Nup214对人细胞的RNA干扰会导致NE处Nup358的强烈减少。 Nup88和Nup214在NPC上显示出相互依赖性，并且不受缺少Nup358的影响。这些数据表明Nup88和Nup214介导Nup358与NPC的连接。我们表明出口受体CRM1在NE的细胞质面上的定位是Nup358依赖的，并代表其空状态。同样，去除Nup358会导致核出口依赖信号的核出口明显减少。我们建议Nup358提供一个用于快速拆卸CRM1出口复合物的平台和一个用于将CRM1空回收到细胞核中的结合位点。

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