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首页> 外文期刊>Molecular and Cellular Biology >Translational Regulation of Nuclear Gene COX4 Expression by Mitochondrial Content of Phosphatidylglycerol and Cardiolipin in Saccharomyces cerevisiae
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Translational Regulation of Nuclear Gene COX4 Expression by Mitochondrial Content of Phosphatidylglycerol and Cardiolipin in Saccharomyces cerevisiae

机译：酿酒酵母中磷脂酰甘油和心磷脂线粒体含量对核基因COX4表达的翻译调控

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摘要

Previous results indicated that translation of four mitochondrion-encoded genes and one nucleus-encoded gene (COX4) is repressed in mutants (pgs1Δ) of Saccharomyces cerevisiae lacking phosphatidylglycerol and cardiolipin. COX4 translation was studied here using a mitochondrially targeted green fluorescence protein (mtGFP) fused to the COX4 promoter and its 5′ and 3′ untranslated regions (UTRs). Lack of mtGFP expression independent of carbon source and strain background was established to be at the translational level. The translational defect was not due to deficiency of mitochondrial respiratory function but was rather caused directly by the lack of phosphatidylglycerol and cardiolipin in mitochondrial membranes. Reintroduction of a functional PGS1 gene under control of the ADH1 promoter restored phosphatidylglycerol synthesis and expression of mtGFP. Deletion analysis of the 5′ UTRCOX4 revealed the presence of a 50-nucleotide fragment with two stem-loops as a cis-element inhibiting COX4 translation. Binding of a protein factor(s) specifically to this sequence was observed with cytoplasm from pgs1Δ but not PGS1 cells. Using HIS3 and lacZ as reporters, extragenic spontaneous recessive mutations that allowed expression of His3p and β-galactosidase were isolated, which appeared to be loss-of-function mutations, suggesting that the genes mutated may encode the trans factors that bind to the cis element in pgs1Δ cells.
机译：先前的结果表明，在酿酒酵母的突变体（ pgs1 Δ）中，四个线粒体编码基因和一个核编码基因（ COX4 ）的翻译受阻。缺乏磷脂酰甘油和心磷脂。使用融合到 COX4 启动子及其5'和3'非翻译区（UTR）的线粒体靶向绿色荧光蛋白（mtG​​FP），研究了 COX4 的翻译。缺乏独立于碳源和菌株背景的 mtGFP 表达被确定为处于翻译水平。翻译缺陷不是由于线粒体呼吸功能的缺乏，而是直接由于线粒体膜中磷脂酰甘油和心磷脂的缺乏而引起的。在 ADH1 启动子控制下重新引入功能性 PGS1 基因可恢复磷脂酰甘油的合成和 mtGFP 的表达。 5'UTR COX4 的缺失分析表明，存在一个带有两个茎环的50个核苷酸的片段，这是 cis 抑制< em> COX4 翻译。在 pgs1 Δ细胞质中观察到蛋白因子与该序列的特异性结合，而在 PGS1 细胞中未观察到。使用 HIS3 和 lacZ 作为报告基因，分离了允许His3p和β-半乳糖苷酶表达的外源性自发性隐性突变，这似乎是功能丧失的突变，表明突变的基因可能编码与 pgs1 Δ细胞中的 cis 元素结合的 trans 因子。

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