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首页> 外文期刊>Molecular and Cellular Biology >DNA Polymerase δ Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension
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DNA Polymerase δ Is Preferentially Recruited during Homologous Recombination To Promote Heteroduplex DNA Extension

机译：在同源重组过程中优先招募DNA聚合酶δ，以促进异源双链DNA延伸

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摘要

DNA polymerases play a central role during homologous recombination (HR), but the identity of the enzyme(s) implicated remains elusive. The pol3-ct allele of the gene encoding the catalytic subunit of DNA polymerase δ (Polδ) has highlighted a role for this polymerase in meiotic HR. We now address the ubiquitous role of Polδ during HR in somatic cells. We find that pol3-ct affects gene conversion tract length during mitotic recombination whether the event is initiated by single-strand gaps following UV irradiation or by site-specific double-strand breaks. We show that the pol3-ct effects on gene conversion are completely independent of mismatch repair, indicating that shorter gene conversion tracts in pol3-ct correspond to shorter extensions of primed DNA synthesis. Interestingly, we find that shorter repair tracts do not favor synthesis-dependent strand annealing at the expense of double-strand-break repair. Finally, we show that the DNA polymerases that have been previously suspected to mediate HR repair synthesis (Polε and Polη) do not affect gene conversion during induced HR, including in the pol3-ct background. Our results argue strongly for the preferential recruitment of Polδ during HR.
机译：DNA聚合酶在同源重组（HR）中起着核心作用，但是所牵涉的酶的身份仍然难以捉摸。编码DNA聚合酶δ（Polδ）催化亚基的基因的 pol3-ct 等位基因突显了该聚合酶在减数分裂HR中的作用。现在，我们探讨在体细胞内HR期间Polδ的普遍作用。我们发现 pol3-ct 会影响有丝分裂重组过程中的基因转换通道长度，无论该事件是由紫外线照射后的单链缺口还是特定于位点的双链断裂引发的。我们显示， pol3-ct 对基因转化的影响与失配修复完全无关，这表明 pol3-ct 中较短的基因转化道对应于引物DNA合成的较短扩展。有趣的是，我们发现较短的修复段不利于合成依赖的链退火，而以双链断裂修复为代价。最后，我们证明先前被怀疑介导HR修复合成的DNA聚合酶（Polε和Polη）在诱导的HR期间，包括在 pol3-ct 背景中，均不影响基因转化。我们的结果有力地证明了在HR期间优先招募Polδ。

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