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Transcriptional Activity Affects the H3K4me3 Level and Distribution in the Coding Region

机译：转录活动影响H3K4me3水平和编码区域中的分布。

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Histone lysine methylation and CpG DNA methylation contribute to transcriptional regulation. We have shown previously that dimethylated and trimethylated forms of histone H3 at lysine 4 (H3K4me2 and H3K4me3) are primarily depleted from CpG-methylated DNA regions by using patch-methylated stable episomes (minichromosomes) in human cells. This effect on H3K4me2 is clearly not linked to the transcriptional activity in the methylated DNA region; however, transcriptional activity may play a role in the presence of H3K4me3. Here, we present clear evidence of the impact of transcriptional activity on the overall level of H3K4me3 in the coding region and the lack of impact on H3K4me2. Our data also demonstrate the influence of transcriptional activity on the distribution of H3K4me3 and H3K4me2, but not that of total H3, in the 5′ end of the coding region relative to the 3′ end. The nature of the promoter (viral or endogenous) affects H3K4me3 much more than it affects H3K4me2, suggesting a potential fundamental difference in the recruitment of methyltransferase for H3K4 trimethylation.
机译：组蛋白赖氨酸甲基化和CpG DNA甲基化有助于转录调控。先前我们已经证明，通过在人细胞中使用补丁甲基化的稳定附加体（微型染色体），赖氨酸4（H3K4me2和H3K4me3）处的组蛋白H3的二甲基化和三甲基化形式主要从CpG-甲基化的DNA区域中耗尽。对H3K4me2的这种影响显然与甲基化DNA区域中的转录活性无关。但是，转录活性可能在H3K4me3的存在下起作用。在这里，我们提供了转录活性对编码区域中H3K4me3总体水平的影响以及对H3K4me2缺乏影响的明确证据。我们的数据还证明了转录活性对编码区5'端相对于3'端的H3K4me3和H3K4me2的分布的影响，但不对总H3的分布的影响。启动子的性质（病毒的或内源的）对H3K4me3的影响远大于对H3K4me2的影响，这表明H3K4三甲基化的甲基转移酶募集方面存在潜在的根本差异。

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