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首页> 外文期刊>Molecular and Cellular Biology >Coordinate regulation of ribosomal component synthesis in Acanthamoeba castellanii: 5S RNA transcription is down regulated during encystment by alteration of TFIIIA activity.
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Coordinate regulation of ribosomal component synthesis in Acanthamoeba castellanii: 5S RNA transcription is down regulated during encystment by alteration of TFIIIA activity.

机译：棘形棘阿米巴核糖体成分合成的协调调控：在包裹过程中，TFIIIA活性的改变下调了5S RNA的转录。

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摘要

Transcription of large rRNA precursor and 5S RNA were examined during encystment of Acanthamoeba castellanii. Both transcription units are down regulated almost coordinately during this process, though 5S RNA transcription is not as completely shut down as rRNA transcription. The protein components necessary for transcription of 5S RNA and tRNA were determined, and fractions containing transcription factors comparable to TFIIIA, TFIIIB, and TFIIIC, as well as RNA polymerase III and a 3'-end processing activity, were identified. Regulation of 5S RNA transcription could be recapitulated in vitro, and the activities of the required components were compared. In contrast to regulation of precursor rRNA, there is no apparent change during encystment in the activity of the polymerase dedicated to 5S RNA expression. Similarly, the transcriptional and promoter-binding activities of TFIIIC are not altered in parallel with 5S RNA regulation. TFIIIB transcriptional activity is unaltered in encysting cells. In contrast, both the transcriptional and DNA-binding activities of TFIIIA are strongly reduced in nuclear extracts from transcriptionally inactive cells. These results were analyzed in terms of mechanisms for coordinate regulation of rRNA and 5S RNA expression.
机译：在棘形棘阿米巴包囊过程中检查了大的rRNA前体和5S RNA的转录。尽管5S RNA转录不如rRNA转录完全关闭，但在此过程中两个转录单位几乎都协同下调。确定了5S RNA和tRNA转录所必需的蛋白质成分，并鉴定了包含与TFIIIA，TFIIIB和TFIIIC相当的转录因子的片段，以及RNA聚合酶III和3'末端加工活性。 5S RNA转录的调节可以在体外概括，并比较了所需成分的活性。与前体rRNA的调节相反，包囊过程中专用于5S RNA表达的聚合酶活性没有明显变化。同样，TFIIIC的转录和启动子结合活性不会与5S RNA调节同时改变。在进入细胞中，TFIIIB转录活性未改变。相反，在转录无活性细胞的核提取物中，TFIIIA的转录和DNA结合活性均大大降低。从协调调节rRNA和5S RNA表达的机制方面分析了这些结果。

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