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首页> 外文期刊>Molecular and Cellular Biology >Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp70 promoter in vivo.
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Role of chromatin and Xenopus laevis heat shock transcription factor in regulation of transcription from the X. laevis hsp70 promoter in vivo.

机译：染色质和非洲爪蟾热休克转录因子在体内调节来自美洲乳杆菌hsp70启动子的转录中的作用。

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摘要

Xenopus laevis oocytes activate transcription from the Xenopus hsp70 promoter within a chromatin template in response to heat shock. Expression of exogenous Xenopus heat shock transcription factor 1 (XHSF1) causes the activation of the wild-type hsp70 promoter within chromatin. XHSF1 activates transcription at normal growth temperatures (18 degrees C), but heat shock (34 degrees C) facilitates transcriptional activation. Titration of chromatin in vivo leads to constitutive transcription from the wild-type hsp70 promoter. The Y box elements within the hsp70 promoter facilitate transcription in the presence or absence of chromatin. The presence of the Y box elements prevents the assembly of canonical nucleosomal arrays over the promoter and facilitates transcription. In a mutant hsp70 promoter lacking Y boxes, exogenous XHSF1 activates transcription from a chromatin template much more efficiently under heat shock conditions. Activation of transcription from the mutant promoter by exogenous XHSF1 correlates with the disappearance of a canonical nucleosomal array over the promoter. Chromatin structure on a mutant hsp70 promoter lacking Y boxes can restrict XHSF1 access; however, on both mutant and wild-type promoters, chromatin assembly can also restrict the function of the basal transcriptional machinery. We suggest that chromatin assembly has a physiological role in establishing a transcriptionally repressed state on the Xenopus hsp70 promoter in vivo.
机译：非洲爪蟾卵母细胞响应热激激活染色质模板中爪蟾hsp70启动子的转录。外源非洲爪蟾热激转录因子1（XHSF1）的表达导致染色质内的野生型hsp70启动子的激活。 XHSF1在正常生长温度（18摄氏度）下激活转录，但热激（34摄氏度）则促进转录激活。体内染色质的滴定导致野生型hsp70启动子的组成型转录。在存在或不存在染色质的情况下，hsp70启动子内的Y盒元件均有助于转录。 Y盒元件的存在阻止了启动子上的规范核小体阵列的组装并促进了转录。在缺少Y框的突变hsp70启动子中，外源XHSF1在热激条件下更有效地激活了染色质模板的转录。外源XHSF1激活突变体启动子转录的过程与启动子上规范核小体阵列的消失有关。缺少Y框的hsp70突变型启动子上的染色质结构可以限制XHSF1的进入;然而，在突变型和野生型启动子上，染色质组装也可以限制基础转录机制的功能。我们建议染色质装配体在非洲爪蟾hsp70启动子体内建立转录抑制状态时具有生理作用。

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