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首页> 外文期刊>Molecular and Cellular Biology >Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase
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Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase

机译：在S期建立裂变酵母Eso1p才能建立姐妹染色单体的内聚力

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Sister chromatid cohesion is essential for cell viability. We have isolated a novel temperature-sensitive lethal mutant namedeso1-H17 that displays spindle assembly checkpoint-dependent mitotic delay and abnormal chromosome segregation. At the permissive temperature, the eso1-H17 mutant shows mild sensitivity to UV irradiation and DNA-damaging chemicals. At the nonpermissive temperature, the mutant is arrested in M phase with a viability loss due to a failure to establish sister chromatid cohesion during S phase. The lethal M-phase arrest phenotype, however, is suppressed by inactivation of a spindle checkpoint. Theeso1 + gene is not essential for the onset and progression of DNA replication but has remarkable genetic interactions with those genes regulating the G1-S transition and DNA replication. The N-terminal two-thirds of Eso1p is highly homologous to DNA polymerase η of budding yeast and humans, and the C-terminal one-third is homologous to budding yeast Eco1p (also called Ctf7p), which is required for the establishment of sister chromatid cohesion. Deletion analysis and determination of the mutation site reveal that the function of the Eco1p/Ctf7p-homologous domain is necessary and sufficient for sister chromatid cohesion. On the other hand, deletion of the DNA polymerase η domain in Eso1p increases sensitivity to UV irradiation. These results indicate that Eso1p plays a dual role during DNA replication. The C-terminal region acts to establish sister chromatid cohesion, and the N-terminal region presumably catalyzes translesion DNA synthesis when template DNA contains lesions that block regular DNA replication.
机译：姐妹染色单体内聚对于细胞活力至关重要。我们已经分离出一种新型的温度敏感致死突变体，名为 eso1-H17 ，该突变体显示纺锤体装配检查点依赖性有丝分裂延迟和异常染色体分离。在允许的温度下， eso1-H17 突变体对紫外线辐射和破坏DNA的化学物质表现出中等敏感性。在不允许的温度下，由于未能在S期建立姐妹染色单体的内聚力，突变体被捕集到M期，丧失了活力。但是，通过禁用主轴检查点可以抑制致命的M期停滞表型。 eso1 + 基因对于DNA复制的开始和发展不是必需的，但与调节G​​ 1 -S的那些基因具有显着的遗传相互作用。过渡和DNA复制。 Eso1p的N端三分之二与发芽酵母和人类的DNA聚合酶η高度同源，C端的三分之二与发芽酵母Eco1p（也称为Ctf7p）同源，这是建立姐妹的必需条件染色单体凝聚力。缺失分析和突变位点的确定表明，Eco1p / Ctf7p同源结构域的功能对于姐妹染色单体的内聚是必要和充分的。另一方面，Eso1p中DNA聚合酶η结构域的缺失增加了对紫外线辐射的敏感性。这些结果表明，Eso1p在DNA复制过程中起着双重作用。当模板DNA包含可阻止规则DNA复制的损伤时，C端区域可建立姐妹染色单体的内聚力，N端区域可催化跨病变DNA的合成。

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