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首页> 外文期刊>Molecular and Cellular Biology >Mmf1p, a Novel Yeast Mitochondrial Protein Conserved throughout Evolution and Involved in Maintenance of the Mitochondrial Genome
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Mmf1p, a Novel Yeast Mitochondrial Protein Conserved throughout Evolution and Involved in Maintenance of the Mitochondrial Genome

机译：Mmf1p，一种新型酵母线粒体蛋白，在整个进化过程中均保持保守，并参与线粒体基因组的维持

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A novel protein family (p14.5, or YERO57c/YJGFc) highly conserved throughout evolution has recently been identified. The biological role of these proteins is not yet well characterized. Two members of the p14.5 family are present in the yeast Saccharomyces cerevisiae. In this study, we have characterized some of the biological functions of the two yeast proteins. Mmf1p is a mitochondrial matrix factor, and homologous Mmf1p factor (Hmf1p) copurifies with the soluble cytoplasmic fraction. Δmmf1cells lose mitochondrial DNA (mtDNA) and have a decreased growth rate, while Δhmf1 cells do not display any visible phenotype. Furthermore, we demonstrate by genetic analysis that Mmf1p does not play a direct role in replication and segregation of the mtDNA. rho+ Δmmf1 haploid cells can be obtained when tetrads are directly dissected on medium containing a nonfermentable carbon source. Our data also indicate that Mmf1p and Hmf1p have similar biological functions in different subcellular compartments. Hmf1p, when fused with the Mmf1p leader peptide, is transported into mitochondria and is able to functionally replace Mmf1p. Moreover, we show that homologous mammalian proteins are functionally related to Mmf1p. Human p14.5 localizes in yeast mitochondria and rescues the Δmmf1-associated phenotypes. In addition, fractionation of rat liver mitochondria showed that rat p14.5, like Mmf1p, is a soluble protein of the matrix. Our study identifies a biological function for Mmf1p and furthermore indicates that this function is conserved between members of the p14.5 family.
机译：最近发现了在整个进化过程中高度保守的新型蛋白质家族（p14.5，或YERO57c / YJGFc）。这些蛋白质的生物学作用尚未很好地表征。酵母酿酒酵母中存在p14.5家族的两个成员。在这项研究中，我们已经表征了两种酵母蛋白的某些生物学功能。 Mmf1p是线粒体基质因子，同源Mmf1p因子（Hmf1p）与可溶性细胞质部分共纯化。 Δ mmf1 细胞会丢失线粒体DNA（mtDNA）并降低生长速率，而Δ hmf1 细胞则没有任何可见的表型。此外，我们通过遗传分析证明Mmf1p在mtDNA的复制和分离中没有直接作用。当将四分体直接切成含有不可发酵碳源的培养基时，可以获得rho + Δ mmf1 单倍体细胞。我们的数据还表明，Mmf1p和Hmf1p在不同的亚细胞区室中具有相似的生物学功能。 Hmf1p与Mmf1p前导肽融合后，被转运到线粒体中，并能够在功能上替代Mmf1p。此外，我们表明，同源的哺乳动物蛋白在功能上与Mmf1p有关。人p14.5定位于酵母线粒体中，并拯救与Δ mmf1 相关的表型。此外，对大鼠肝线粒体进行分级分离显示，与Mmf1p一样，大鼠p14.5是基质的可溶性蛋白。我们的研究确定了Mmf1p的生物学功能，并且进一步表明该功能在p14.5家族成员之间是保守的。

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